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OALib Journal期刊
ISSN: 2333-9721
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油松叶绿体dna间隔序列特点及系统学意义
DOI: 10.3724/SP.J.1145.2010.00143, PP. 143-147
Keywords: 油松,cpdna间隔序列,系统进化,分子标记
Abstract:
对全国分布区12个天然油松种群进行取样，测定了叶绿体dnatrnt-trnl、trns-trng、trnl-trnf基因间隔序列，分析了序列碱基组成特点及在松科系统分类中的意义.测序结果显示，3个片段碱基序列均富含a/t，长度依次为448bp、636bp和421bp，所测片段在种群水平上十分保守，没有发现具有种内鉴别意义的碱基变异.但此片段适合于松科属间和种间的系统学分析，运用phylip软件构建松科植物的邻接系统树，支持松科分为两个亚科的分类系统，拓扑结构表明松属与银杉属的关系最近.由于叶绿体dna序列长度适中，扩增和测序较为容易，适合作为研究松科植物系统进化较为理想的分子标记.图2表2参24
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