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OALib Journal期刊
ISSN: 2333-9721
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不同光照下盐生杜氏藻光捕获蛋白基因lhcb及叶绿素a合成酶基因cao的表达变化
DOI: 10.3724/SP.J.1145.2010.00018, PP. 18-22
Keywords: 光系统ⅱ,lhcⅱ,lhcb基因家族,表达量,叶绿素加氧酶
Abstract:
由于光捕获天线蛋白（lhcⅱ）编码基因（lhcb）家族同源性较高，以往研究只能从总体上反映家族基因的变化，对各个编码基因的表达研究较少.本研究以光合单细胞生物盐生杜氏藻（dunaliellasalina）为材料，应用实时pcr的方法，系统研究了lhcb基因家族成员在光环境转换过程中的表达变化.结果表明，当由正常培养光环境转移到高光条件下时，lhcb基因家族成员的表达整体上受抑制，但各个基因的表达变化趋势随时间变化不尽相同.在强光照下，lhcb1、lhcb2.1、lhcb2.23h时有最低的表达积累量，而lhcb3却有较高的表达量.细胞转入黑暗环境后，各个基因的转录水平升高，变化趋势基本相同，在3h时均有较高的表达量，只有lhcb2.1在1h时的表达量最高.叶绿素加氧酶编码基因cao的变化与lhcb3基本相同.lhcⅱ蛋白量在高光下降低，在黑暗条件下升高.叶绿素含量在高光条件下明显下降，叶绿素a/b值升高，黑暗条件下则相反.图4参19
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