OALib Journal期刊
ISSN: 2333-9721
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甲苯降解菌jb-1的分离、鉴定及其降解特性研究
DOI: 10.3724/SP.J.1145.2010.00118, PP. 118-121
Keywords: 甲苯,生物降解,假单胞菌属,甲苯双加氧酶,邻苯二酚2,3双加氧酶,代谢途径
Abstract:
从南京石化废水处理池的活性污泥中分离到1株能以甲苯为唯一碳源生长的细菌,命名为jb-1.根据其生理生化特征和16srdna(genbankaccessionno.eu869278)序列相似性分析,将该菌株鉴定为假单胞菌属(pseudomonassp.).该菌株在18h内能完全降解346.4mg/l的甲苯.降解甲苯的最适温度为30℃,ph为7.0,降解速率与初始接种量呈正相关.甲苯降解途径是通过甲苯双加氧酶形成顺甲苯二氢二醇,再经邻苯二酚2,3双加氧酶开环降解.图2参22
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