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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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一株产脂肪酶嗜盐菌的鉴定及耐盐机理
DOI: 10.3724/SP.J.1145.2010.00100, PP. 100-103
Keywords: 菌种鉴定,halorubrumaidingense,脂肪酶,极端嗜盐菌,四氢嘧啶
Abstract:
从采自内蒙古锡林浩特地区盐湖的样品中筛选到产脂肪酶嗜盐菌jnph-3，该菌株可在含有1.5~5mol/lnacl的培养基上生长，最适生长温度45℃，最适ph7~9.5.经形态学分析、生理生化实验及16srdna鉴定，jnph-3菌株为halorubrumaidingense，属于极端嗜盐菌.jnph-3菌株在生长过程中专一性依赖na+，对cl-依赖性较小，k+浓度变化对菌体生长无明显影响.高效液相色谱分析表明，jnph-3菌株在液体复合培养基中能够产生四氢嘧啶，且与环境中盐浓度呈负相关，说明jnph-3体内除存在产生四氢嘧啶的机制来维持渗透平衡，还有其它机制来维持细胞正常生长.图3表3参14
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