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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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用甜菜夜蛾增殖马尾松毛虫质型多角体病毒的研究
DOI: 10.3724/SP.J.1145.2010.00084, PP. 84-90
Keywords: 马尾松毛虫,质型多角体病毒,甜菜夜蛾,替代宿主
Abstract:
以甜菜夜蛾（spodopteraexigua）作为替代宿主增殖马尾松毛虫质型多角体病毒（dpcpv）的研究结果表明，dpcpv在甜菜夜蛾体内连续传代3次后得到的dpcpv-se3与dpcpv具有相同的电泳图谱；多角体和病毒粒子在电镜下观察，未发现形态上的明显变化.生测结果表明，dpcpv-se3与dpcpv回接松毛虫试验，其半数致死浓度分别为0.92×103pib/ml和1.44×103pib/ml，二者毒力差异不显著.以甜菜夜蛾增殖dpcpv，多角体产量达到2.34×108pib/头.用dpcpv-se3和dpcpv的s7和s10片段分别进行同源性序列分析，结果没有明显变化.认为利用甜菜夜蛾增殖dpcpv用于生物防治具有可行性.图6表2参22
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