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OALib Journal期刊
ISSN: 2333-9721
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一株抗青枯假单胞菌放线菌的筛选、鉴定及发酵条件优化
DOI: 10.3724/SP.J.1145.2010.00079, PP. 79-83
Keywords: 青枯假单胞菌,抗生链霉菌,抑菌谱,发酵条件
Abstract:
从河南中牟土壤中筛选出一株对青枯假单胞菌（pseudomonassolanacearum）有较强拮抗作用的放线菌zm-16，它对几种常见病原细菌和真菌都有较强的抑制效果，其发酵液抗菌谱较广.通过菌株形态特征、培养特性、生理生化特性、化学特征研究以及16srrna序列分析，初步确定菌株zm-16为链霉菌属的抗生链霉菌（streptomycesantibioticus）.通过发酵优化实验，确定该菌株的最佳发酵配方为：蔗糖8%、大豆粉5%、nano31%、nacl0.2%、caco30.4%、k2hpo40.08%；最佳发酵条件为：发酵培养基初始ph值7.0，30℃，恒温摇床200r/min，培养6d，抑菌效果最好.图2表6参19
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