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OALib Journal期刊
ISSN: 2333-9721
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宿根甘蔗根际土壤细菌多样性分析中培养法与非培养法比较研究
DOI: 10.3724/SP.J.1145.2011.00742, PP. 742-746
Keywords: 甘蔗,根际土壤微生物,细菌群落,ardra,多样性分析
Abstract:
采用培养法和非培养法提取甘蔗根际土壤微生物的总dna，基于通用引物pcr扩增构建两种方法的细菌16srdna文库，并进行核糖体dna扩增片段限制性内切酶分析（ardra），通过部分克隆序列测定构建细菌克隆文库的系统发育树，进而对基于传统的细菌平板培养法和直接提取总细菌dna的非培养法进行比较分析.结果显示，非培养法文库的香侬？威纳指数、辛普森指数、丰富度分别为4.94、0.998、28.91，均高于培养法文库中相应的多样性参数（分别为4.27、0.996、16.79），均一度数值都＞0.95.结合统计学数据和序列测定信息，反映出甘蔗根际土壤存在着丰富的细菌多样性，但平板培养法所展现的多样性低于直接提取法，表明前者存在着很大的局限性，而非培养法文库中主要是未培养微生物的16srdna序列，这从一个侧面反映了土壤样品中未培养微生物占很大比例.因此，在土壤微生物群落研究中，必须结合新的分子生态学技术手段，如采用直接提取总dna的方法进行研究，才能比较全面地认识土壤微生物多样性.图3表3参18
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