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OALib Journal期刊
ISSN: 2333-9721
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亚硝酸盐急性胁迫对中华绒螯蟹幼体相关免疫指标和应激蛋白（hsp70）表达的影响
DOI: 10.3724/SP.J.1145.2011.00688, PP. 688-693
Keywords: 亚硝酸盐胁迫,中华绒螯蟹,超氧化物歧化酶,丙二醛,血细胞总数,hsp70
Abstract:
为探讨亚硝酸盐急性胁迫对中华绒螯蟹机体免疫力的影响，以及机体在胁迫过程中的应对策略，分别测定了幼蟹（平均体重3.25g）在10、20、30、40mgl-1亚硝酸盐暴露组和对照组中血细胞总数（totalhemocytecounts，thc）、超氧化物歧化酶（superoxidedismutase，sod）活力、丙二醛（malodiadehyde，mda）含量以及热休克蛋白70（heatstressprotein70，hsp70）表达量在受胁迫2d内的动态变化.结果表明：1）低浓度亚硝酸盐（10mgl-1）胁迫组thc在胁迫3h时显著高于对照组，肝胰腺中sod活性在胁迫3h和6h时亦显著提高，表现出一定的“毒物兴奋效应”.2）但随着暴露时间的延长和亚硝酸盐浓度的升高，幼蟹thc减少，肝胰腺sod活性下降，而mda含量升高.3）对照组中有少量的hsp70表达.幼蟹经10、20mgl-1亚硝酸盐处理后，鳃组织中hsp70表达量在胁迫6h后达到最大，而经30、40mgl-1亚硝酸盐处理后，hsp70表达量在胁迫3h后达到最大.各浓度组所诱导的hsp70最大表达量与水中亚硝酸盐的浓度呈正相关.以上结果说明亚硝酸盐胁迫可通过加剧组织中脂质过氧化的方式导致机体免疫力下降，肝胰腺中mda的含量变化可作为亚硝酸盐胁迫的有效评价指标之一，而hsp70表达量的变化可作为早期预警指标应用于亚硝酸盐胁迫评价中.图2表1参36
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