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OALib Journal期刊
ISSN: 2333-9721
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bt肠毒素基因与plcr基因的相关性及肠毒素对小鼠的急性毒性
DOI: 10.3724/SP.J.1145.2011.00418, PP. 418-421
Keywords: 苏云金芽胞杆菌,肠毒素基因,调控基因,急性毒性
Abstract:
用pcr方法检测溶血肠毒素bl、肠毒素t和肠毒素s三种肠毒素基因（hbla、bect、ents）及毒素调控基因plcr在30株苏云金芽胞杆菌株（bt）中的分布.结果表明，含有hbla基因、ents基因、bect基因及plcr基因片段的bt菌株分别占66.7%、70%、70%和73.3%.其中，含有plcr基因的菌株都至少含有一种肠毒素基因，不含肠毒素基因的菌株也未检测到plcr基因，说明plcr基因与肠毒素基因有着密切的关系.用3个bt菌株wb9、hd2和hd9（都含有hbla、bect、ents和plcr基因片段，hd2和hd9经rpla与tecra肠毒素检测试剂盒检测具有高滴度）对小白鼠进行口服急性毒性试验.结果表明，所有3种供试菌株的发酵上清液对小白鼠行为和健康没有明显影响，对内部器官(心、肝、肺等)也没有产生病理现象.图5表3参14
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