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OALib Journal期刊
ISSN: 2333-9721
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慢生根瘤菌dg的分离、鉴定及其与降香黄檀的共生关系
DOI: 10.3724/SP.J.1145.2011.00379, PP. 379-383
Keywords: 根瘤菌,降香黄檀,结瘤,固氮作用,共生关系
Abstract:
从大果紫檀根瘤中分离到一株慢生型根瘤菌dg，通过其形态特征观察、保守基因序列分析以及生物学特性研究等方面进行种类鉴定，通过结瘤试验了解dg与降香黄檀的共生关系，并探讨其共生固氮能力.16srrna、nifh和dnak系统发育结果表明，该菌株与bradyrhizobiumelkanii同源性最高.菌株dg与b,elkaniiusda76t在碳源利用、抗生素抗性、耐受nacl等生物学特性上基本一致，而且还能在ph10.0下良好生长.结瘤试验显示，菌株dg能在降香黄檀根部结瘤；接种dg的降香黄檀幼苗植株的株高、干重以及全氮含量（p<0.05）显著高于对照处理，表明dg能与降香黄檀形成良好的共生关系，且具有高效的固氮能力.图4表2参27
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