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OALib Journal期刊
ISSN: 2333-9721
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青稞b-hordein基因表达载体构建及对小麦的遗传转化
DOI: 10.3724/SP.J.1145.2011.00364, PP. 364-368
Keywords: 青稞,b组醇溶蛋白,载体构建,转基因,小麦,gateway?
Abstract:
利用从具有特殊b-hordein亚基组成的青藏高原青稞材料中克隆的b-hordein编码基因（sl60）和小麦高分子量谷蛋白亚基胚乳特异表达启动子pglu1dx构建了真核表达载体pcb2007-sl60.通过农杆菌介导对普通小麦进行遗传转化，共获得227株再生植株，经pcr鉴定和转化片段测序验证，最终获得5株阳性植株.研究为进一步分析b-hordein基因在小麦背景中的遗传和表达，以及对小麦加工特性的影响奠定了基础.图5表2参21
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