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OALib Journal期刊
ISSN: 2333-9721
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麻疯树成熟胚乳再生植株及其气孔分析
DOI: 10.3724/SP.J.1145.2011.00353, PP. 353-358
Keywords: 麻疯树,愈伤组织诱导,不定芽分化,叶片分化,生根,植株再生,气孔分析
Abstract:
对麻疯树成熟胚乳进行组织培养获得胚乳再生植株，并对其气孔进行分析.麻疯树成熟胚乳在25℃、12h光照条件下培养7d愈伤组织诱导完成，2,4-d浓度为2.0mgl-1的ms培养基愈伤诱导效果最好，诱导率达89.29%.愈伤组织在含bap的改良培养基上培养至黄绿色后转入分化培养基，在含iaa0.25mgl-1和zt1.5mgl-1的wpm培养基上不定芽分化率达32.50%.将分化的不定芽从愈伤组织上剥离后转入含iba、bap和ga3的培养基上进行芽伸长培养.取胚乳不定芽叶片接种在含iba0.1mgl-1、bap0.5mgl-1和tdz0.5mgl-1的ms培养基上诱导生芽后，再转入含iaa0.25mgl-1、kt0.5mgl-1、bap1.0mgl-1和ga30.25mgl-1的培养基上进行丛生芽的诱导，成芽率为85.2%.这些芽在含0.1mgl-1iba的1/2ms培养基上生根，大约有37.5%的芽生了根，平均有5.2条根系形成.与母本植株相比，再生的胚乳植株保卫细胞更大，且气孔密度减小.图2表6参24
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