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一株高浸磷嗜酸氧化硫硫杆菌的分离与鉴定

DOI: 10.3724/SP.J.1145.2011.00326, PP. 326-329

Keywords: 嗜酸氧化硫硫杆菌,菌种筛选,16srdna,生物浸磷,黄铁矿

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Abstract:

从矿山土样中分离到一株嗜酸的浸磷矿细菌hy-01,观察其形态并研究其培养特征.结果显示,该菌株为革兰氏阴性、短杆状运动细菌,菌体长1~2μm,宽0.5~0.7μm,能在ph1.5~8.0的范围内生长,最适生长温度为37℃,最适ph为1.5~3.0.该菌株可以kno3、nh4no3、(nh4)2co3、nh4h2po4、(nh4)2so4以及蛋白胨为氮源,以元素硫和黄铁矿中的硫为能源,不能利用有机物质以及硫代硫酸钠、硫酸亚铁等无机物为能源生长.形态、生理生化特性研究以及16srdna序列分析表明,该菌株为嗜酸氧化硫硫杆菌.浸矿实验结果表明其具有很高的磷矿浸出能力.图6表1参18

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