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OALib Journal期刊
ISSN: 2333-9721
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嗜热自养甲烷杆菌的抗逆分子机制
DOI: 10.3724/SP.J.1145.2011.00307, PP. 307-310
Keywords: 古菌,嗜热自养甲烷杆菌,二硫键异构酶,胁迫
Abstract:
为揭示嗜热厌氧古菌适应极端环境的分子机理，分别在不同温度（50℃，60℃，65℃和70℃）培养嗜热自养甲烷杆菌，利用real-timert-pcr研究其二硫键异构酶（mtpdi）mrna的表达情况.结果显示，mtpdimrna在上述情况下都有表达，但当温度偏离菌体最适生长温度（65℃），表达量会提高，在50℃和70℃时达到最大.mtpdimrna的表达量在50℃下培养时是最适生长温度（65℃）时的10.3倍，在60℃培养时是最适生长温度（65℃）时的2.7倍，在70℃培养时是最适生长温度（65℃）时的9.3倍.说明mtpdi的表达与温度相关，当偏离最适生长温度时mtpdimrna表达上调，以帮助体内蛋白质正确折叠.研究表明mtpdi具有协助嗜热自养甲烷杆菌抵抗外界逆境胁迫的作用.图4参18
| [1] | 7kernr,malkia,holmgrena,richarmeg.chaperonepropertiesofescherichiacolithioredoxinandthioredoxinreductase.biochemj,2003,371:965~972
|
| [2] | 8pandharej,deshpandev.bothchaperoneandisomerasefunctionsofproteindisulfideisomeraseareessentialforaccelerationoftheoxidativerefoldingandreactivationofdimericalkalineproteaseinhibitor.proteinsci,2004,13:2493~2501
|
| [3] | 9vincif,ruoppolom,puccip,freedmanrb,marinog.earlyintermediatesinthepdi-assistedfoldingofribonucleasea.proteinsci,2000,9:525~535
|
| [4] | 10eichlerj,adamsmw.posttranslationalproteinmodificationinarchaea.microbiolmolbiolrev,2005,69:393~425
|
| [5] | 11balchwe,foxge,magrumlj,woesecr,wolfers.methanogens:reevaluationofauniquebiologicalgroup.microbiolrev,1979,43:260~296
|
| [6] | 12guptars,singhb.phylogeneticanalysisof70kdheatshockproteinsequencessuggestsachimericoriginfortheeukaryoticcellnucleus.currbiol,1994,4:1104~1114
|
| [7] | 14bustardk,guptars.thesequencesofheatshockprotein40(dnaj)homologsprovideevidenceforacloseevolutionaryrelationshipbetweenthedeinococcus-thermusgroupandcyanobacteria.jmolevol,1997,45:193~205
|
| [8] | 15macarioaj,demacarioec.thearchaealmolecularchaperonemachine:peculiaritiesandparadoxes.genetics,1999,152:1277~1283
|
| [9] | 16macarioaj,langem,ahringbk,demacarioec.stressgenesandproteinsinthearchaea.microbiolmolbiolrev,1999,63:923~967
|
| [10] | 17demacarioec,macarioaj.molecularbiologyofstressgenesinmethanogens:potentialforbioreactortechnology.advbiochemengbiotechnol,2003,81:95~150
|
| [11] | 18dingx(丁霞),minh(闵航),yangwj(杨卫军).heterologousexpressionandpurificationofmtpdifrommethanothermobacterthermautotrophicuinescherichiacoli.jnanchangunivnatsci(南昌大学学报理科版),2010,34(5):498~502
|
| [12] | 1eckburgpb,lepppw,relmanda.archaeaandtheirpotentialroleinhumandisease.infectimmun,2003,71:591~596
|
| [13] | 2smithdr,doucette-stammla,delougheryc,leeh,duboisj,aldredget,bashirzadehr,blakelyd,cookr,gilbertk,harrisond,hoangl,keaglep,lummw,pothierb,qiud,spadaforar,vicairer,wangy,wierzbowskij,gibsonr,jiwanin,carusoa,bushd,reevejn,etal.completegenomesequenceofmethanobacteriumthermoautotrophicumdeltah:functionalanalysisandcomparativegenomics.jbacteriol,1997,179:7135~7155
|
| [14] | 3javauxej.extremelifeonearth-past,presentandpossiblybeyond.resmicrobiol,2006,157:37~48
|
| [15] | 4wucx(吴初新),liuy(刘毅),mams(马梅生),wanglk(王立坤),hucy(胡成钰).up-regulationofgrasscarpgrp78geneexpressionunderheatshockandpolyi:cstress.chinjapplenvironbiol(应用与环境生物学报),2009,15(6):814~818
|
| [16] | 5chenj,songjl,zhangs,wangy,cuidf,wangcc.chaperoneactivityofdsbc.jbiolchem,1999,274:19601~19605
|
| [17] | 6ferraridm,solinghd.theproteindisulphide-isomerasefamily:unravellingastringoffolds.biochem,1999,339(pt1):1~10
|
| [18] | 13guptars.evolutionofthechaperoninfamilies(hsp60,hsp10andtcp-1)ofproteinsandtheoriginofeukaryoticcells.molmicrobiol,1995,15:1~11
|
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