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OALib Journal期刊
ISSN: 2333-9721
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土壤宏蛋白质组学蛋白质提取方法及其应用
DOI: 10.3724/SP.J.1145.2012.00691, PP. 691-696
Keywords: 土壤微生物,宏蛋白质组学,蛋白质提取方法,应用
Abstract:
土壤宏蛋白质组学在揭示土壤微生物功能、代谢与环境相互作用方面具有广阔的应用前景，但由于土壤样品的特殊性，土壤蛋白质提取步骤是限制土壤宏蛋白质组学大规模应用的瓶颈之一.本文从样品制备、提取方法、影响因素等方面综述了土壤蛋白提取方面的研究进展.一般来说，根据实验目的、蛋白种类及后续研究方法设计相应的分组成收集策略才能取得较好的提取效果.土壤总蛋白、胞内蛋白与胞外蛋白分别有不同的提取方法.总蛋白提取一般采用直接提取法；胞外蛋白不需要裂解；胞内蛋白提取方法有直接提取法和间接提取法等.裂解、浓缩、去除腐殖质的方法以及提取液、ph值的选择等也会影响提取效果.此外，简单介绍了土壤宏蛋白质组学的应用，并对今后的研究工作提出展望.表1参36
| [1] | 5watanabes,yoshikawah.characterizationofneutralphosphatebufferextractablesoilorganicmatterbyelectrophoresisandfractionationusingultrafiltration.soilsci&plantnutr,2007,53(5):650~656
|
| [2] | 6harnerm,ramseyp,rilligm.proteinaccumulationanddistributioninfloodplainsoilsandriverfoam.ecollett,2004,7(9):829~836
|
| [3] | 7ogunseitano.soilproteomics:extractionandanalysisofproteinsfromsoils.nuclacids&proteinssoil,2006,8:95~115
|
| [4] | 8ramrj,verberkmoesnc,thelenmp,tysongw,bakerbj,blakerc,shahm,hettichrl,banfieldjf.communityproteomicsofanaturalmicrobialbiofilm.science,2005,308(5730):1915~1920
|
| [5] | 9benndorfd,balckegu,harmsh,vonbergenm.functionalmetaproteomeanalysisofproteinextractsfromcontaminatedsoilandgroundwater.ismej,2007,1(3):224~234
|
| [6] | 10giagnonil,magherinif,landil,taghavis,modestia,binil,nannipierip,vanderlelied,renellag.extractionofmicrobialproteomefromsoil:potentialandlimitationsassessedthroughamodelstudy.eurjsoilsci,2011,62(1):74~81
|
| [7] | 11linwx(林文雄),xiongj(熊君),linhf(林辉锋),theextractionofsoilproteinandtheseparationmethodofintracellularprotein.chinapatentofinvention(中国发明专利),101531708a,2009
|
| [8] | 12tayloreb,williamsma.microbialproteininpoil:influenceofextractionmethodandcamendmentonextractionandrecovery.microbecol,2010,59(2):390~399
|
| [9] | 13wilmesp,bondpl.theapplicationoftwo-dimensionalpolyacrylamidegelelectrophoresisanddownstreamanalysestoamixedcommunityofprokaryoticmicroorganisms.environmicrobiol,2004,6(9):911~920
|
| [10] | 14choureyk,janssonj,verberkmoesn,shahm,chavarriakl,tomlm,brodieel,hettichrl.directcellularlysis/proteinextractionprotocolforsoilmetaproteomics.jproteomeres,2010,9(12):6615~6622
|
| [11] | 15singletoni,merringtong,colvans,delahuntyjs.thepotentialofsoilprotein-basedmethodstoindicatemetalcontamination.applsoilecol,2003,23(1):25~32
|
| [12] | 16abramf,gunniglee,o’flahertyv.optimisationofproteinextractionand2-deformetaproteomicsofmicrobialcommunitiesfromanaerobicwastewatertreatmentbiofilms.electrophoresis,2009,30(23):4149~4151
|
| [13] | 17murasea,yonedam,uenor,yonebayashik.isolationofextracellularproteinfromgreenhousesoil.soilbiol&biochem,2003,35(5):733~736
|
| [14] | 18criquets,farnetam,ferree.proteinmeasurementinforestlitter.biol&fertilsoils,2002,35(5):307~313
|
| [15] | 23haoc(郝纯),liuqh(刘庆华),yangjs(杨俊仕),lixd(李旭东).metaproteomics:explorationofthefunctionsofmicrobialecosystems.chinjapplenvironbiol(应用与环境生物学报),2008,14(2):270~275
|
| [16] | 24liuhh(刘虎虎),tiany(田云),luxy(卢向阳),fangj(方俊).metaproteomics:anewstrategyforstudyingmicrobialcommunity.jmicrobiol(微生物学杂志),2010,30(5):88~92
|
| [17] | 25benndorfd,vogtc,jehmlichn,schmidty,thomash,woffending,shevchenkoa,richnowhh,vonbergenm.improvingproteinextractionandseparationmethodsforinvestigatingthemetaproteomeofanaerobicbenzenecommunitieswithinsediments.biodegradation,2009,20(6):737~750
|
| [18] | 26khalilib,nilin,nourbakhshf,sharifnabib,khademih.doescultivationinfluencethecontentandpatternofsoilproteins?soil&tillageres,2010,111(2):162~167
|
| [19] | 1bastidaf,morenojl,nicolasc,hernandezt,garciac.soilmetaproteomics:areviewofanemergingenvironmentalscience.significance,methodologyandperspectives.eurjsoilsci,2009,60(6):845~859
|
| [20] | 2lipsond,näsholmt.theunexpectedversatilityofplants:organicnitrogenuseandavailabilityinterrestrialecosystems.oecologia,2001,128(3):305~316
|
| [21] | 3chenc,xuz,zhangs,keayp.solubleorganicnitrogenpoolsinforestsoilsofsubtropicalaustralia.plant&soil,2005,277(1):285~297
|
| [22] | 4yurt(于仁涛),gaopj(高培基),hanl(韩黎),huangly(黄留玉).strategyandapplicationofmetaproteomics.chinjbiotech(生物工程学报),2009(7):961~967
|
| [23] | 19masciandarog,maccic,donis,masertibe,leoacb,ceccantib,wellingtone.comparisonofextractionmethodsforrecoveryofextracellularbeta-glucosidaseintwodifferentforestsoils.soilbiol&biochem,2008,40(9):2156~2161
|
| [24] | 20weil(魏力),yangcy(杨成运),liyg(李友国).newapproachesandstrategiesforstudyingfunctionsofenvironmentmicrobialcommunities.actaecolsin(生态学报),2008,28():4424~4429
|
| [25] | 21niuz(牛泽).metaproteome�anewapproachforstudyingmicrobialecologyfunction.microbiology(微生物学通报),2007,34(4):804~807
|
| [26] | 22chengnn(成妮妮).metaproteomics:newtechnologyofstudyinggeneexpressioninmicrobialcommunities.microbiology(微生物学通报),2007,34(2):347~349
|
| [27] | 27lacerdac.environmentalproteomeprofilingappliedtothestudyofpolybacterialmetalresistanceandadaptation:[phdthesis].fortcollins:coloradostateuniversity,2008
|
| [28] | 28lacerdacmr,choelh,reardonkf.metaproteomicanalysisofabacterialcommunityresponsetocadmiumexposure.jproteomeres,2007,6(3):1145~1152
|
| [29] | 29schulzewx,gleixnerg,kaiserk,guggenbergerg,mannm,schulzeed.aproteomicfingerprintofdissolvedorganiccarbonandofsoilparticles.oecologia,2005,142(3):335~343
|
| [30] | 30schulzewx.proteinanalysisindissolvedorganicmatter:whatproteinsfromorganicdebris,soilleachateandsurfacewatercantellus-aperspective.biogeosciences,2005,2(1):75~86
|
| [31] | 31liptonms,pa?a-toli?l,andersonga,andersondj,auberrydl,battistajr,dalymj,fredricksonj,hixsonkk,kostandarithesh.globalanalysisofthedeinococcusradioduransproteomebyusingaccuratemasstags.procnatacadsciusa,2002,99(17):11049
|
| [32] | 32ramr,verberkmoesn,thelenm,tysong,bakerb,blaker,shahm,hettichr,banfieldj.communityproteomicsofanaturalmicrobialbiofilm.science,2005,308(5730):1915
|
| [33] | 33pengj,eliasje,thoreencc,lickliderlj,gygisp.evaluationofmultidimensionalchromatographycoupledwithtandemmassspectrometry(lc/lc-ms/ms)forlarge-scaleproteinanalysis:theyeastproteome.jproteomeres,2003,2(1):43~50
|
| [34] | 34liuwt,janssonjk.metaproteomics:techniquesandapplications.environmentalmolecularmicrobiology.norfolk:caisteracademicpress,2010
|
| [35] | 35jehmlichn,schmidtf,taubertm,seifertj,vonbergenm,richnowhh,vogtc.comparisonofmethodsforsimultaneousidentificationofbacterialspeciesanddeterminationofmetabolicactivitybyprotein�basedstableisotopeprobing(protein�sip)experiments.rapidcommunmassspectrom,2009,23(12):1871~1878
|
| [36] | 36jehmlichn,schmidtf,taubertm,seifertj,bastidaf,vonbergenm,richnowhh,vogtc.protein-basedstableisotopeprobing.natprotocols,2010,5(12):1957~1966
|
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