全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

用改进的trap法测定树木端粒酶活性

DOI: 10.3724/SP.J.1145.2012.00682, PP. 682-686

Keywords: 树木,端粒酶活性,trap,sybrgreeni染色

Full-Text   Cite this paper   Add to My Lib

Abstract:

trap法是一种常用的测定端粒酶活性的方法,但并不太适合测定木本植物的端粒酶活性.因此以油松针叶为实验材料,对trap法进行改进.参照前人的研究通过对比实验证明,在端粒酶提取过程中将peg8000加入上清液,可获得高效的端粒酶.在此基础上,对pcr先导引选择ts、模板浓度选择100ng,最终获得油松针叶高质量的端粒酶pcr产物.应用sybrgreeni替代银染液对电泳凝胶进行染色,获得条带清晰、重复性好的电泳结果.通过对油松针叶、银杏叶片、胡杨愈伤组织、沙冬青悬浮细胞等4种木本植物实验材料进行测定,证明该改进的技术体系可能是适合于木本植物端粒酶活性测定的稳定的、灵敏度高的可行方法.图5表1参17

References

[1]  1rihak,mcknighttd,fajkusj,vyskotb,shippende.analysisoftheg-overhangstructuresonplanttelomeres:evidencefortwodistincttelomerearchitectures.plantj,2000,23(5):633~641
[2]  2fajkusj,fulneckoraj.plantcellsexpresstelomeraseactivityupontransfertocallusculture,withoutextensivelychangingtelomerelengths.molgengenet,1998,260(5):470~474
[3]  miloslavaf,janaf,fajkusj.recoveryoftobaccocellsfromcadmiumstressisaccompaniedbydnarepairandincreasedtelomeraseactivity.jexpbot,2002,53(378):2151~2158
[4]  greidercw,blackburneh.identificationofaspecifictelomereterminaltransferaseactivityintetrahymenaextracts.cell,1985,43:405~413
[5]  kimnw,piatyszekma,prowsekr,harleycb,westmd,hopl,coviellogm,wrightwe,weinrichsl,shayjw.specificassociationofhumantelomeraseactivitywithimmortalcellsandcancer.science,1994,266:2011~2015
[6]  wenjm,sunlb,zhengmh.anon-isotopicmethodforthedetectionoftelomeraseactivityintumortissue:trap-siverstainingassay.molpatbol,1998,51(2):110~112
[7]  thurnherd,knererb,formanekm,kornfehj.non-radioactivesemiquantitativetestingfortheexpressionlevelsoftelomeraseactivityinheadandnecksquamouscellcarcinomasmaybeindicativeforbiologicaltumourbehavior.actaotolaryngol,1998,118:423~427
[8]  weilx(卫立辛),guoyj(郭亚军),yanzl(闫振林),shijx(施军霞),shenf(沈峰),xietp(谢天培),cuizf(崔贞福),wumc(吴孟超).detectionofhumentelomeraseactvitybytelomerasetrap-elisaassay.chinjoncol(中华肿瘤杂志),1998,20(4):264~266
[9]  yangxf,wangy,luojp.transformationoflea3geneintodendrobiumcandidumwall.exlindlforenhancingitssalttolerance.chinjapplenvironbiol(应用与环境生物学报),2010,16(5):622~626
[10]  liuxc(刘小川),liangyh(梁永恒),chenb(陈波).quantitativeanalysisfortelomeraseactivityinrice.chinabiotechnol(中国生物工程杂志),2007,27(5):45~49
[11]  rihak,fajkusj,sirokyj.developmentalcontroloftelomerelengthsandtelomeraseactivityinplants.plantcell,1998,10(10):1691~1698
[12]  fajkusj,fulneckovaj,hulanovam,berkovak,riham,matyasekr.plantcellsexpresstelomeraseactivityupontransfertocallusculture,withoutextensivelychangingtelomerelengths.molgengenet,1998,260(5):470~474
[13]  fitzgerldms,mcknighttd,dorothye,shippende.characterizationanddevelopmentalpatternsoftelomeraseexpressioninplants.pnas,1996,93:14422~14427
[14]  songh,liud,lifl,luh.season-andage-associatedtelomeraseactivityinginkgobilobal.molbiolrep,2011,38:1799~1805
[15]  zhangll,katsunorit,kazuos,hideot.thetelomeraseinhibitortelomestatininducestelomereshorteningandcelldeathinarabidopsis.bba,2005,1763(2006):39~44
[16]  dallatca,macielrmb,pinheirona,andradejad,toledosrc,villall,ceruttijm.trap-silverstaining,ahighlysensitiveassayformeasuringtelomeraseactivityintumortissueandcelllines.brazjmedbiolres,2002,35:65~68
[17]  fujitam,tomitas,ueday,fujimorit.gelstainingmethodsdordetectionoftelomeraseactivitywiththetelomericrepeatamplificationprotocol(trap)assay.molpathol,1998,51:342

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133