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鲜甘薯发酵生产燃料乙醇中的降粘工艺

DOI: 10.3724/SP.J.1145.2012.00661, PP. 661-666

Keywords: 鲜甘薯,品种,降粘,纤维素酶,燃料乙醇,工业化生产

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Abstract:

鲜甘薯高浓度发酵生产燃料乙醇的瓶颈之一是醪液粘度高,容易堵塞管路,严重影响工业化生产和增加能源消耗,同时也会降低乙醇发酵效率.为解决此问题,进行了添加降粘酶系及其作用条件优化研究,结果如下:1)确定最适降粘酶系为四川禾本生物工程有限公司的纤维素酶,粘度由1.7×104mpa.s降到8.8×102mpa.s,并且降低了生产成本;2)确定降粘酶作用前高温处理条件:110℃,20min;3)最适降粘酶对不同品种鲜甘薯高浓度发酵的降粘效果表明降粘酶对大部分品种鲜甘薯降粘效果较好,粘度均约为1.0×103mpa.s以下,最低粘度只有2.7×102mpa.s,粘度下降率均在95%以上;4)在确定最适降粘酶系和其作用前高温条件后,将其应用于工业化生产,加入降粘酶2h后发酵醪液的粘度由1.8×105mpa.s下降到2.7×103mpa.s,发酵后终粘度仅为7.9×102mpa.s,发酵时间仅为23h,乙醇浓度达到10.56%(v/v),进一步验证了该降粘酶系应用于工业化鲜甘薯燃料乙醇生产的实际意义.表8参19

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