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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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多拷贝表达转鲑鱼降钙素基因酵母的急性、亚急性毒性
DOI: 10.3724/SP.J.1145.2012.00630, PP. 630-634
Keywords: 转鲑鱼降钙素酵母,小鼠,大鼠,急性毒性,亚急性毒性
Abstract:
口服转鲑鱼降钙素基因酵母为鲑鱼降钙素的应用开辟了一条新的途径.为评价转鲑鱼降钙素基因酵母的安全性，将转基因酵母分别灌胃昆明种小鼠和wistar大鼠进行急性毒性实验（7d）、亚急性毒性实验（8周）.急性毒性实验结果表明灌喂转基因酵母对小鼠无致死效应（ld50>10000mg/kg）；亚急性毒性实验中高（2.0g/kg）、低（0.5g/kg）剂量组动物的一般状况、体重、主要脏器系数、血液学指标及血液生化指标与各对照组比较，均未见明显差异（p>0.05），组织病理切片检查未发现病理性变化.实验结果说明转鲑鱼降钙素基因酵母不会对实验动物造成不良影响，是安全无毒的.图3表3参16
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