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OALib Journal期刊
ISSN: 2333-9721
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丹参提取液对球形红细菌菌体蛋白及几种酶的影响
DOI: 10.3724/SP.J.1145.2012.00623, PP. 623-629
Keywords: 光合细菌,球形红细菌,丹参提取液,聚丙烯酰胺凝胶电泳,同工酶,生物转化
Abstract:
为探索球形红细菌对丹参的生物转化机理，采用非变性聚丙烯酰胺凝胶电泳（n-page）分别对丹参水提液、醇提液、醇水提液培养后球形红细菌菌体及纯球形红细菌（prs）菌体蛋白质（pro）、酯酶（est）、细胞色素氧化酶（cod）、超氧化物歧化酶（sod）和过氧化物酶（pod）进行分析，比较用丹参提取液培养前后球形红细菌菌体蛋白及4种酶的同工酶变化.结果表明：丹参提取液培养前后球形红细菌菌体蛋白及4种酶的同工酶谱带差别较大，同工酶的电泳迁移率、活性、所表达同工酶的数目及分布均有差异，培养d2~6蛋白及酶表达量变化最大，d14~20基本稳定.研究表明丹参能诱导球形红细菌生成新的蛋白质及酶，亦可抑制某些蛋白质和酶的合成；这些蛋白和酶可能参与了丹参化学成分的生物转化.图4参22
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