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OALib Journal期刊
ISSN: 2333-9721
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偶发分枝杆菌发酵断甾醇侧链积累9α-羟基雄烯二酮
DOI: 10.3724/SP.J.1145.2014.07022, PP. 256-262
Keywords: 生物降解,甾醇侧链,定向筛选,分枝杆菌,9a-oh-ad,半微量制备实验
Abstract:
利用偶发分枝杆菌（mycobacteriumfortuitum）cicc10279发酵断甾醇侧链进行定向菌株筛选；对选出的具有较高甾醇降解活性的菌株，借助tlc法开展多批次生物降解实验过程研究；并重点对积累9a-羟基雄烯二酮（9a-oh-ad）化合物的菌株稳定性及转化过程进行考察；在此基础上利用选出的22号菌株分别进行底物为胆固醇和植物甾醇的半微量制备实验.结果显示，分离的样品具有高纯度，hplc分析为95.7%；tlc、hplc、ms、1hnmr、13cnmr、i.r.等光谱数据分析结构确证其为9a-oh-ad；半微量制备实验以胆固醇或植物甾醇为底物，9a-oh-ad重量收得率分别可达34.0%和30.8%.本研究可能为高效含卤（氟，氯）皮质激素药物工业生产提供了一种很有用处的中间体.
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