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OALib Journal期刊
ISSN: 2333-9721
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内酯型双乙酰槐糖脂对肿瘤细胞k562的抑制作用
DOI: 10.3724/SP.J.1145.2014.08005, PP. 377-380
Keywords: 内酯型双乙酰槐糖脂,抗肿瘤,k562,细胞坏死
Abstract:
糖脂是一类由酵母菌产生的糖脂类生物表面活性剂.双乙酰化的内酯型槐糖脂具有很好的药物学活性，研究其抗肿瘤活性对于拓宽槐糖脂的应用领域具有重要意义.通过mtt法倒置相差显微镜观察、荧光染色、dna电泳研究内酯型双乙酰槐糖脂对肿瘤细胞k562的抑制作用.结果显示：槐糖脂能够抑制肿瘤细胞k562的增殖，作用24h和48h，ic50值分别为31.70μg/ml和29.80μg/ml.细胞形态发生了明显的变化，细胞胀大，细胞膜破裂，细胞内容物外溢、细胞解体.细胞核出现收缩、碎裂和溶解.dna发生随机断裂，电泳时出现弥散性涂抹状条带.本研究表明，内酯型双乙酰槐糖脂能够抑制肿瘤细胞k562的增殖，细胞坏死是抑制k562细胞增殖的主要原因.
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