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OALib Journal期刊
ISSN: 2333-9721
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甘蔗光合系统ⅰ亚基o基因的克隆与表达分析
DOI: 10.3724/SP.J.1145.2014.09033, PP. 208-214
Keywords: 甘蔗,psao基因,生物信息学,荧光定量pcr
Abstract:
光合系统ⅰ亚基o（photosystemⅰsubunito，psao）是光合系统ⅰ中的蛋白亚基，在两个光合系统之间平衡激发能方面起着重要的作用.为研究psao基因的结构和功能，对甘蔗（saccharumofficinaruml.）叶片全长cdna文库进行测序，获得光合系统ⅰ亚基o基因的全长cdna序列，命名为scpsao（genbankaccessionnumber：kf714498）.生物信息学分析表明，该基因全长708bp，开放阅读框435bp，编码144个氨基酸；该基因所编码的蛋白定位于叶绿体基质，无信号肽，为疏水性非分泌碱性蛋白，二级结构多为无规则卷曲，含有pjn00046家族的保守结构域，参与能量新陈代谢及脂肪酸新陈代谢.同时，该基因在不同物种间具有较强的保守性，其中与同属c4植物的同源性较c3植物高.荧光定量pcr分析结果表明，甘蔗scpsao基因在叶片中的相对表达量最高，具有一定的组织特异性；在氯化钠（nacl）、聚乙二醇（peg）、氯化铜（cucl2）、脱落酸（aba）、水杨酸（sa）和茉莉酸甲酯（meja）等外源胁迫下，其表达量均呈下调趋势，且以peg胁迫下的下调表现最为明显.这些结果表明这几种外源胁迫可能抑制甘蔗scpsao基因的转录水平表达，为其进一步功能验证以及在甘蔗基因工程中的应用积累了基础资料.
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