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OALib Journal期刊
ISSN: 2333-9721
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工业酿酒酵母菌株kf-7对发酵抑制物的耐受性
DOI: 10.3724/SP.J.1145.2014.08009, PP. 248-255
Keywords: 燃料乙醇,工业酿酒酵母,发酵抑制物,批次发酵
Abstract:
木质纤维素原料预处理过程中产生的抑制物是燃料乙醇发酵的一大障碍，要求工业酿酒酵母菌株具有优秀的抑制物耐受能力.利用平板培养和批次发酵两种方式系统评价了弱酸抑制物（乙酸、甲酸、乙酰丙酸）、呋喃类抑制物[糠醛和5-羟甲基糠醛（hmf）]、酚类抑制物（香草醛、丁香醛、苯酚）对工业酿酒酵母菌株kf-7生长和发酵的影响.结果显示，菌株kf-7在批次发酵时细胞生长对抑制物的耐受性优于平板培养.低浓度的抑制物虽然对菌株的生长有一定的抑制作用，但对乙醇的产生具有一定的促进作用；高浓度抑制物显著抑制了菌株的生长，降低了葡萄糖的代谢速率，抑制了乙醇的产生.菌株kf-7对甲酸耐受能力强于乙酸，对乙酰丙酸的耐受能力较弱.在平板生长评价中，糠醛对菌株生长的抑制作用强于hmf，但在批次发酵过程中hmf的抑制作用强于糠醛；该菌株代谢糠醛的能力强于代谢hmf的能力.香草醛对菌株的抑制作用最强，丁香醛相对较弱.在秸秆水解液中，菌株kf-7也表现出良好的乙醇发酵性能.菌株kf-7无论在单一抑制物、混合抑制物或实际水解液条件下发酵，均能达到较高的乙醇收率.本研究表明，菌株kf-7适用于纤维素原料燃料乙醇工业化生产过程.
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