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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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不同根瘤菌中突变cysdn基因对硫酸盐同化途径的影响
DOI: 10.3724/SP.J.1145.2014.10020, PP. 242-247
Keywords: cysdn,硫酸盐同化途径,费氏中华根瘤菌,苜蓿中华根瘤菌,大豆慢生根瘤菌,同源双交换,植株实验
Abstract:
硫是生物体必须的元素，根瘤菌结瘤因子的硫化修饰与其宿主范围和识别效率有着重要关系，实验室前期研究中突变费氏中华根瘤菌的cysdn基因后，突变体不能利用硫酸盐生长，这与sinorhizobiumsp.br816的cysd基因突变后的现象不同，两株菌的硫酸盐同化途径中可能存在差异.为探讨不同根瘤菌cysdn基因对硫酸盐同化途径的影响，本研究利用同源交换的方法构建费氏中华根瘤菌wgf03、费氏中华根瘤菌hn01、苜蓿中华根瘤菌14500及大豆慢生根瘤菌15606的cysdn突变体，δcysdn-wgf03、δcysdn-hn01、δcysdn-14500和cysnr-15606，对突变体的硫源利用情况和植株结瘤情况进行研究.结果发现，δcysdn-wgf03和δcysdn-hn01失去利用硫酸盐的能力；δcysdn-14500能微弱地利用硫酸盐生长，生长量约为野生菌株的1/3左右；cysnr-15606对硫酸盐的利用与野生菌株相比无明显差别.植株实验结果显示，δcysdn-wgf03、δcysdn-hn01和δcysdn-14500在平均瘤数、平均瘤重、平均植株干重和固氮酶活性方面均表现出显著降低，而突变体的回补体能够恢复硫酸盐的利用能力及与植株的共生固氮能力.这说明cysdn基因在费氏中华根瘤菌wgf03、费氏中华根瘤菌hn01和苜蓿中华根瘤菌14500硫酸盐同化途径中起着关键影响，而该基因在大豆慢生根瘤菌15606的影响并不明显.本研究表明，cysdn基因在不同根瘤菌中所起的作用不同，不同根瘤菌的硫酸盐同化方式也存在差异.
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