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OALib Journal期刊
ISSN: 2333-9721
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四川地区结瘤大豆根际土壤中紫云英、苜蓿和三叶草根瘤菌的多样性分析
DOI: 10.3724/SP.J.1145.2014.08021, PP. 234-241
Keywords: 根瘤菌,大豆根际土壤,紫云英,苜蓿,三叶草
Abstract:
为了解四川地区结瘤大豆根际土壤是否存在与紫云英、苜蓿和三叶草共生结瘤的根瘤菌及其多样性，从四川盆地采集不同种植模式下结瘤大豆根际土壤样品31份，利用紫云英、苜蓿和三叶草进行盆栽捕获试验以获得共生根瘤，从根瘤中分离纯化出根瘤菌，对其进行表型和分子鉴定；并将根瘤菌回接到紫云英、苜蓿、三叶草和大豆根际以检测根瘤菌的寄主范围.在捕获实验中，苜蓿和三叶草分别在6个土壤样品中共生结瘤，而紫云英只在2个土样中共生结瘤，并从共生根瘤中分离出14株根瘤菌；16srdna基因序列的相似性分析表明这14株根瘤菌均属于根瘤菌属（rhizobium），且16srdna基因的系统发育树揭示它们分属于根瘤菌属的不同种；这14株根瘤菌在回接实验中都只能让捕获豆科植物结瘤，而不能让其他3种豆科植物结瘤.本研究结果表明，四川地区结瘤大豆根际土壤中的紫云英根瘤菌（rhizobiumastragali）、苜蓿根瘤菌（r.meliloti）和三叶草根瘤菌（r.leguminosarumvar.trifolii）资源较少，其与大豆根瘤菌（bradyrhizobiumjaponicum）分属于不同的属，且根瘤菌的回接实验进一步证明了根瘤菌的寄主专一性.
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