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OALib Journal期刊
ISSN: 2333-9721
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水库贫营养异养硝化-好氧反硝化菌sxf14的脱氮特性
DOI: 10.3724/SP.J.1145.2014.10002, PP. 201-207
Keywords: 贫营养,异养硝化,好氧反硝化,16srrna,水库
Abstract:
为利用生物强化法降低微污染源水中的氮素，从水库沉积物中筛选到一株好氧反硝化细菌sxf14.通过扫描电镜和16srrna序列分析，鉴定其为不动杆菌属（acinetobactersp.），命名为acinetobactersp.sxf14.同时，对该菌株脱氮特性进行研究，并将其接种到c/n（总有机碳与总氮的比值）为1.2的微污染水库源水中，以探究其对实际源水总氮的去除效果.结果显示：sxf14能以硝酸盐和亚硝酸盐为唯一氮源进行好氧反硝化.反应48h后，no3--n和no2--n的去除率分别达74.84(±0.86)%和40.52(±1.49)%，tn去除率最高达到65.07(±1.56)%和41.33(±0.98)%；在以nh4cl为氮源的异养硝化系统内，该菌在48h内使nh4+-n浓度由3.73(±0.08)mg/l降到1.28(±0.20)mg/l，氨氮去除率达到65.63(±1.39)%.72h内，微污染水库源水的tn浓度由2.46(±0.02)mg/l降到1.68(±0.01)mg/l，去除率达到31.7(±0.14)%.因此，菌株acinetobactersp.sxf14具有反硝化能力，能承受较低的碳氮比，降低微污染源水中的氮素，本研究可为微污染水体的菌剂修复技术提供科学依据.
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