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OALib Journal期刊
ISSN: 2333-9721
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家蝇中肠细菌群落组成分析
DOI: 10.3724/SP.J.1145.2013.00800, PP. 800-804
Keywords: 家蝇,中肠,细菌群落,16srrna基因,克隆文库
Abstract:
家蝇（muscadomestica）是重要的医学媒介昆虫，是许多病原体传播的机械媒介和生物媒介.为了研究家蝇中肠内微生物的群落结构，了解家蝇在疾病传播中的作用，构建了家蝇中肠细菌的16srrna基因克隆文库.文库包括88条序列，分别属于22个操作分类单元（otus），其中12个otus属于变形菌门（proteobacteria），9个otus属于壁厚菌门（firmicutes），1个otu属于拟杆菌门（bacteroidetes）roteobacteria有10个otus，roteobacteria和epsinoproteobacteria各1个otu，roteobacteria和deltaproteobacteria.壁厚菌门中，6个otus属于lactobacillales，1otu属于clostridia，2otus属于bacillales.文库中gammaproteobacteria有71条序列，占总序列数的80.7%，是优势种类.文库中的肠杆菌属（enterobacter）、克雷伯菌属（klebsiella）、普罗威登斯菌属（providencia）、变形杆菌属（proteus）、气单胞菌属（aeromonas）和葡萄球菌属（staphylococcus）中的许多种类都是条件致病菌和致病菌.研究结果将有助于了解家蝇在疾病传播中的作用.图3表1参29
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