|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
内皮素-1刺激对血管平滑肌过氧化物酶体增殖物激活受体γ表达的作用
, PP. 1140-1144
Keywords: ppar-γ,内皮素-1(et-1),血管平滑肌
Abstract:
目的研究et-1对血管平滑肌过氧化物酶体增殖物激活受体γ(ppar-γ)表达的影响以及与血管平滑肌增殖的关系。方法以成年16周wistar大鼠为研究对象,采用主动脉血管平滑肌原代培养技术,培养大鼠腹主动脉血管平滑肌1~3代,然后加入et-1进行刺激,使用rt-pcr和westernblot技术观察et-1作用后0、12、24、48、72h以及不同浓度et-1刺激48h后ppar-γmrna和蛋白表达变化,同时用mtt法检测血管平滑肌(vsmcs)增殖。结果et-1刺激后12h,ppar-γ在mrna和蛋白水平表达上未见明显变化(p>0.05),而24h时出现了表达轻度下调,与对照组(无et-1刺激)差异具有显著性(p<0.05),48~72h下降更加明显,与对照组相比差异具有高度显著性(p<0.01)。不同浓度et-1刺激48h后,随着浓度的增加,ppar-γ表达减低,各组相比具有显著性差异(p<0.01)。而血管平滑肌的增殖在12h就出现,且随着时间的延长,增殖增强。而且随着et-1浓度的增加,vsmcs增殖增加。结论et-1在引起vsmcs增殖的同时,可引起ppar-γ在mrna和蛋白水平的表达减低,提示et-1导致的vsmcs增值可能与ppar-γ有一定的关系。
| [1] | shichirim,yokokuram,marumof,etal.endothelin-linhibitsapoptosisofvascularsmoothmusclecellsinducedbynitricoxideandserumdeprivationviamapkinasepathway[j].arteriosclerthrombvasc,2000,20(4):989-97.
|
| [2] | hinterhuberl,graziadeiiw,kahlercm,etal.endothelin-receptorantagonisttreatmentofportopulmonaryhypertension[j].clingastroenterolhepatol,2004,2(11):1039-42.
|
| [3] | hayneswg,ferrocj,o’kanekp,etal.systemicendothelinreceptorblockadedecreasesperipheralvascularresistanceandbloodpressureinhumans[j].circulation,1996,93(10):1860-70.
|
| [4] | ihlingc,bohrmannb,schaeferhe,etal.endothelin-1andendothelinconvertingenzyme-1inhumanatherosclerosis-noveltargetsforpharmacotherapyinatherosclerosis[j].currvascpharmacol,2004,2(3):249-58.
|
| [5] | kellydp.thepleiotropicnatureofthevascularppargeneregulatorypathway[j].circres,2001,89(11):935-7.
|
| [6] | verrethw,dekeyzerd,pelatm,etal.weightloss-associatedinductionofperoxisomeproliferator-activatedreceptor-alphaandperoxisomeproliferator-activatedreceptor-gammacorrelatewithreducedatherosclerosisandimprovedcardiovascularfunctioninobeseinsulin-resistantmice[j].circulation,2004,110(20):3259-69.
|
| [7] | marxn,duezh,fruchartjc,etal.peroxisomeproliferatoractivatedreceptorsandatherogenesis:regulatorsofgeneexpressioninvascularcells[j].circres,2004,94(9):1168-78
|
| [8] | castrilloa,tontonozp.pparsinatherosclerosis:theclotthickens[j].jclininvest,2004,114(11):1538-40.
|
| [9] | iglarzm,touyzrm,amirif,etal.effectofperoxisomeproliferatoractivatedreceptor-alphaand-gammaactivatorsonvascularremodelinginendothelin-dependenthypertension[j].arteriosclerthrombvascbiol,2003,23(1):45-51.
|
| [10] | dzauvj,braun-dullaeusrc,seddingdg.vascularproliferationandatherosclerosis:newperspectivesandtherapeuticstrategies[j].natmed,2002,8(11):1249-56.
|
| [11] | merkusd,dunckerdj,chilianwm.metabolicregulationofcoronaryvasculartone:roleofendothelin-1[j].amjphysiolheartcirephysiol,2002,283(5):1915-192.
|
| [12] | oltert,wiselygb,westins,etal.ligandbindingandco-activatorassemblyoftheperoxisomeproliferator-activatedreceptor-gamma[j].nature,1998,395(6698):137-43.
|
| [13] | schiffrinel.peroxisomeproliferator-activatedreceptorsandcardiovascularremodeling[j].amjphysiolheartcircphysiol,2005,288(3):h1037-43.
|
| [14] | hasdaid,holmesdrjr,garrattkn,etal.mechanicalpressureandstretchreleaseendothelin-1fromhumanatheroscleroticcoronaryarteriesinvivo[j].circulation,1997,95(2):357-62.
|
| [15] | bergerj,mollerde.themechanismsofactionofppars[j].annurevmed,2002,339:953-95.
|
| [16] | delerivep,martin-nizardf,chinettig,etal.peroxisomeproliferatoractivatedreceptoractivatorsinhibitthrombin-inducedendothelin-1productioninhumanvascularendothelialcellsbyinhibitingtheactivatorprotein-1signalingpathway[j].circres,1999,85(5):394-402.
|
| [17] | wakinos,kintscheru,kims,etal.peroxisomeproliferatoractivatedreceptorgammaligandsinhibitretinoblastomaphosphorylationandg1stransitioninvascularsmoothmusclecells[j].jbiolchem,275(29):435-41.
|
| [18] | kintscheru,lyoncj,lawre.angiotensinⅱ,ppar-gammaandatherosclerosis[j].frontbiosci,2004,9:359-69.
|
| [19] | mauricioj.reginato,samuell,etal.prostaglandinspromoteandblockadipogenesisthroughopposingeffectsonperoxisomeproliferator-activatedreceptor[j].jbiol,273(4),1855-8.
|
| [20] | liujj,huangrw,lindj,etal.angiotensinⅱandendothelin-1regulatemapkinasesthroughdifferentredox-dependentmechanismsinhumanvascularsmoothmusclecells[j].jhypertens,2004,22(6):1141-9.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
