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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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b型链球菌数量感应通路测定及luxs缺失突变株的构建
, PP. 1135-1139
Keywords: b型链球菌,数量感应通路,luxs分子,生物发光测定
Abstract:
目的对gbs中可能存在的ai-2数量感应通路进行测定,构建数量感应相关分子luxs缺失突变株并对其基因型进行确认。方法培养gbs至不同d(λ)值,利用v.harveyibb170作为报告菌株,对gbs诱导生物发光的能力进行测定,然后寻找gbs中luxs同源基因,通过同源重组法在gbs中构建luxs缺失突变株,southern杂交分析确证。结果gbs中的某种分泌成份可在报告菌株中诱导生物发光活性,提示gbs中存在ai-2数量感应通路,在gbs中发现了luxs同源基因并成功地构建了luxs缺失突变株。结论本研究为探索luxs在gbs中ai-2数量感应通路中的重要性及gbs毒力研究建立了细菌模型。
| [1] | gibbsrs,schrags,schuchata.perinatalinfectionsduetogroupbstreptococci[j].obstetgynecol,2004,104(5pt1):1062-76.
|
| [2] | tettelinh,masignaniv,cieslewiczmj,etal.completegenomesequenceandcomparativegenomicanalysisofanemerginghumanpathogen,serotypevstreptococcusagalactiae[j].procnatlacadsciusa,2002,99(19):12391-6.
|
| [3] | glaserp,rusniokc,buchrieserc,etal.genomesequenceofstreptococcusagalactiae,apathogencausinginvasiveneonataldisease[j].molmicrobiol,2002,45(6):1499-13.
|
| [4] | wagnerve,gillisrj,iglewskibh.transcriptomeanalysisofquorum-sensingregulationandvirulencefactorexpressioninpseudomonasaeruginosa[j].vaccine,2004,6(22suppl1):s15-20.
|
| [5] | xavierkb,basslerbl.regulationofuptakeandprocessingofthequorum-sensingautoinducerai-2inescherichiacoli[j].jbacteriol,2005,187(1):238-48.
|
| [6] | henkejm,basslerbl.threeparallelquorum-sensingsystemsregulategeneexpressioninvibrioharveyi[j].jbacteriol,2004,186(20):6902-14.
|
| [7] | xavierkb,basslerbl.luxsquorumsensing:morethanjustanumbersgame[j].curropinmicrobiol,2003,6(2):191-97.
|
| [8] | tagame,semmelhackjl,basslerbl.theluxs-dependentautoinducerai-2controlstheexpressionofanabctransporterthatfunctionsinai-2uptakeinsalmonellatyphimurium[j].molmicrobiol,2001,42(3):777-93.
|
| [9] | sperandiov,melliesjl,nguyenw,etal.quorumsensingcontrolsexpressionofthetypeⅲsecretiongenetranscriptionandproteinsecretioninenterohemorrhagicandenteropathogenicescherichiacoli[j].procnatlacadsciusa,1999,96(26):15196-201.
|
| [10] | joness,yub,baintonnj,etal.theluxautoinducerregulatestheproductionofexoenzymevirulencedeterminantsinerwiniacarotovoraandpseudomonasaeruginosa[j].emboj,1993,12(6):2477-82.
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