|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
用乳酸脱氢酶为靶分子的免疫层析技术检测恶性疟原虫
, PP. 761-765
Keywords: 恶性疟原虫,乳酸脱氢酶,单克隆抗体,免疫胶体金层析
Abstract:
目的建立一种免疫胶体金层析(gica)法用于恶性疟原虫的检测。方法采用杂交瘤技术制备恶性疟原虫乳酸脱氢酶(ldhpf)单抗,利用筛选出来的单抗制成诊断恶性疟原虫的免疫层析条,以镜检和pcr法为对照,用gica条检测门诊"四热"病人血样,评价其敏感性和特异性,并对其稳定性进行了初步研究。结果筛选出4株抗ldhpf单抗,间接elisa表明4株单抗仅与恶性疟原虫发生反应,而与间日疟原虫、红细胞蛋白、刚地弓形虫和日本血吸虫不发生交叉反应。western-blot结果显示4株单抗能识别恶性疟原虫相对分子质量约为33000处的虫源蛋白。以镜检法和pcr法为标准,gica条检测恶性疟原虫的敏感性分别为88.37%和86.67%,gica与镜检法的符合率均为91.55%。结论gica检测恶性疟原虫简易快速、灵敏度高,无需特殊仪器设备,有望成为一种恶性疟原虫快速免疫诊断试剂盒。
| [1] | uguenc,rabodonirinam,depinajj,etal.parasight-frapidmanualdiagnostictestofplasmodiuminfection[j].bullworldhealthorgan,1995,73(5):643-9.
|
| [2] | palmercj,lindojf,klaskalawi,etal.evaluationoftheoptimaltestforrapiddiagnosisofplasmodiumvivaxandplasmodiumfalciparummalaria[j].jclinmicrobiol,1998,36(1):203-6.
|
| [3] | kaushaldc,kaushalna,chandrad.monoclonalantibodiesagainstlactatedehydrogenaseofplasmodiumknowlesi[j].indianjexpbiol,1995,33(1):6-11.
|
| [4] | kilianah,mughusueb,kabagambeg,etal.comparisonoftworapidhrp-2baseddiagnosistestsforplasmodiumfaciparum[j].transrsoctropmedhyg,1997,91(6):666-7.
|
| [5] | gomanm,monsb,scaifej.thecompletesequenceofaplasmodiummalariaessurrnageneanditscomparisontootherplasmodiumssurrnagenes[j].molbiochemparasitol,1991,45(2):281-8.
|
| [6] | qarish,goldmanif,pieniazeknj,etal.bloodandsporozoitestage-specificsmallsubunitribosomalrna-encodingrnagenesofthehumanmalariaparasiteplasmodiumvivax[j].gene,1994,150(1):43-9.
|
| [7] | gozarmm,bagnaraas.identificationofababesiabovisgenewithhomologytothesmallsubunitribosomalrnagenefromthe35-kilobasecirculardnaofplasmodiumfalciparum[j].intjparasitol,1993,23(1):145-8.
|
| [8] | barkerrh,banchongaksomt,courvaljm,etal.plasmodiumfalciparumandp.vivax:factorsaffectingsensitivityandspecificityofpcr-baseddiagnosisofmalaria[j].expparasitol,1994,79(1):41-9.
|
| [9] | thepsamarnp,prayoollawongsap,puksupap,etal.theictmalariapf:asimple,rapiddipsticktestforthediagnosisofplasmodiumfalciparummalariaatthethai-myanmarborder[j].southeastasianjtropmedpublichealth,1997,28(4):723-6.
|
| [10] | millerrl,ikrams,armelagosgj,etal.diagnosisofplasmodiumfalciparuminfectionsinmummiesusingtherapidmanualparasight-ftest[j].transrsoctropmedhyg,1994,88(1):31-2.
|
| [11] | laferih,kandelk,pichlerh.falsepositivedipsticktestformalaria[j].nengljmed1997,337(22):1635-6.
|
| [12] | iqbakj,shera,raba.plasmodiumfalciparumhistidine-richprotein2-basedimmunocapturediagnosticassayformalaria:crossreactivitywithrheumatoidfactors[j].jclinmicrobiol,2000,38(3):1184-6.
|
| [13] | maklermt,riesjm,williamsja,etal.parasitelactatedehydrogenaseasanassayforplasmodiumfalciparumdrugsensi-tivity[j].amjtropmedhyg,1993,48(6):739-41.
|
| [14] | maklermt,hinrichsdj.measurementofthelactatedehydrogenaseactivityofplasmodiumfalciparumasanassessmentofparasitemia[j].amjtropmedhyg,1993,48(2):205-10.
|
| [15] | goodyerid,taraschitf.plasmodiumfalciparum:asimple,rapidmethodfordetectingparasiteclonesinmicrotiterplates[j].expparasitol,1997,86(2):158-60.
|
| [16] | bascolk,marquetf,maklermm.plasmodiumfalciparumandplasmodiumvivax:lactatedehydrogenaseactivityanditsapplicationforinvitrodrugsusceptibilityassay[j].expparasitol,1995,80(2):260-71.
|
| [17] | oduolaam,omitowojugo,sowunmia,etal.plasmodiumfalciparum:evaluationoflactatedehydrogenaseinmonitoringtherapeuticresponsestostandardantimalarialdrugsinnigeria[j].expparasitol,1997,87(3):283-9.
|
| [18] | kaushaldc,wattsr,haiders,etal.antibodiestolactatedehydrogenaseofplasmodiumknowlesiarespecifictoplasmodiumspecies[j].immunolinvest,1988,17(6-7):507-16.
|
| [19] | lij,wirtzra,mcconkeyga,etal.transitionofplasmodiumvivaxribosometypescorrespondstosporozoitedifferentiationinthemosquito[j].molbiochemparasitol,1994,65(2):283-9.
|
| [20] | 吴英松,李明,董文其,等恶性疟原虫乳酸脱氢酶的表达及免疫活性鉴定[j].中国寄生虫学与寄生虫病杂志,2001,19(2):80-3.
|
| [21] | watersap,mccutchantf.rapid,sensitivediagnosisofmalariabasedonribosomalrna[j].lancet,1989,1(8651):1343-6.
|
| [22] | iqbalj,shera,hirapr,etal.comparisonoftheoptimaltestwithpcrfordiagnosisofmalariainimmigrants[j].jclinmicrobiol,1999,37(11):3644-6.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
