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OALib Journal期刊
ISSN: 2333-9721
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肺炎克雷伯杆菌对小鼠中性粒细胞表达il-1β和tnf-α的影响
, PP. 805-808
Keywords: 肺炎克雷伯杆菌,中性粒细胞,细胞因子
Abstract:
目的观察肺炎克雷伯杆菌(klebsiellapneumoniae,kle.p)感染对小鼠中性粒细胞细胞因子表达的影响。方法以热灭活的kle.p感染小鼠肺部建立急性肺炎模型,分离并纯化中性粒细胞(pmn),用rt-pcr、westernblotting、elisa等方法检测pmn中tnf-α和il-1β的表达改变。结果kle.p感染促进了早期小鼠中性粒细胞肿瘤坏死因子-α(tnf-α)和白介素-1β(il-1β)的mrna表达及分泌,tnf-αmrna表达在感染6h最高,12h后降低;il-1β的mrna表达在感染12h最强;24h后两者表达均减弱,但仍显著强于对照组。westernblotting结果显示,感染6h后tnf-α、il-1β的蛋白表达量最高,24h后表达明显减少。elisa结果显示,tnf-α分泌量在感染后6h最高,24h后明显降低,但仍较对照组高;il-1β分泌量峰值在感染12h后出现。结论tnf-α可能主要参与小鼠感染热灭活kle.p早期的pmn免疫防御功能;而il-1β则主要参与感染中后期的pmn免疫防御功能。
| [1] | sunilvr,connoraj,zhouph,etal.activationofadherentvascularneutrophilsinthelungduringacuteendotoxemia[j].respirres,2002,3(1):21.
|
| [2] | 徐江平,杨雪梅.烟碱对白细胞介素1诱导的培养牛脑微血管内皮细胞表达avb3整合素的增强作用[j].第一军医大学学报,2001,21(4):266-9xujp,yangxm.enhancementofil-1β-inducedexpressionofαvβ3integrinbynicotineinculturedbovinecerebralmicrovascularendothelialcells[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2001,21(4):266-9.
|
| [3] | pouliotm,serhancn.lipoxina4andaspirin-triggered15-epi-lxa4inhibittumornecrosisfactor-alpha-initiatedneutrophilresponsesandtrafficking:novelregulatorsofacytokine-chemokineaxisrelevanttoperiodontaldiseases[j].jperiodontalres,1999,34(7):370-3.
|
| [4] | zhangp,summerwr,bagbygj,etal.innateimmunityandpulmonaryhostdefense[j].immunolrev,2000,173(1):39-51.
|
| [5] | bergerony,deslauriersam,ouelletn,etal.influenceofcefodizimeonpulmonaryinflammatoryresponsetoheat-killedklebsiellapneumoniaeinmice[j].antimicrobagentschemother,1999,43(9):2291-4.
|
| [6] | hamasakia,sendof,nakayamak,etal.acceleratedneutrophilapoptosisinmicelackingal-a,asubtypeofthebcl-2-relateda1gene[j].jexpmed,1998,188:1985-92.
|
| [7] | julielh,kleanthisg,xanthopoulos.ccaat/enhancerbindingproteinεiscriticalforeffectiveneutrophil-mediatedresponsetoinflammatorychallenge[j].blood,1999,93(9):3096-105.
|
| [8] | songh,ligw,qianys,etal.modulationofneutrophilcytokinessecretionbyklebsiellapneumoniae[j].compclinpathol,2004,13:14-8.
|
| [9] | chandelns,trzynawc,mcclintockds,etal.roleofoxidantsinnf-kappabactivationandtnf-alphagenetranscriptioninducedbyhypoxiaandendotoxin[j].jimmunol,2000,165(2):1013-21.
|
| [10] | 孙卫民,王惠琴.细胞因子研究方法学[m].北京:人民卫生出版社,1999584-615.
|
| [11] | abrahame,carmodya,shenkarr.neutrophilsasearlyimmunologiceffectorsinhemorrhage-orendotoxemia-inducedacutelunginjury[j].amjphysiollungcellmelphysiol,2000,279(6):l1137-45.
|
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