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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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阿司匹林对血管内皮细胞增殖及磷酸化p44/42mapk表达的抑制作用
, PP. 1013-1015
Keywords: 阿司匹林,内皮细胞,细胞增殖,丝裂素活化蛋白激酶
Abstract:
目的观察阿司匹林对体外培养的血管内皮细胞的增殖和磷酸化p44/42丝裂素活化蛋白激酶(mapk)表达的影响。方法体外培养血管内皮细胞株ecv304,应用1、2、5、10mmol/l的阿司匹林分别作用并设立对照进行比较,采用mts/pes法确定ecv304细胞的增殖状态。利用p44/42磷酸化抗mapk抗体的蛋白免疫印迹法测定磷酸化p44/42mapk蛋白表达。结果各组的细胞增殖率分别为对照组1.533±0.286,阿司匹林1mmol/l组1.253±0.225,2mmol/l组0.953±0.149,5mmol/l组0.708±0.125,10mmol/l组0.459±0.107。统计分析显示低至1mmol/l的阿司匹林仍能明显抑制ecv304细胞的增殖(p<0.05),并呈现剂量依赖性。阿司匹林对ecv304细胞的磷酸化p44/p42mapk表达有显著的抑制作用(p<0.05)。结论阿司匹林对体外培养的血管内皮细胞的增殖和内皮细胞中磷酸化p44/42mapk的表达均有明显的抑制作用。
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