|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
重组幽门螺杆菌过氧化氢酶对结肠粘膜上皮细胞氧化应激的影响
, PP. 1045-1047
Keywords: 重组幽门螺杆菌过氧化氢酶,结肠炎,氧化性应激,氧自由基
Abstract:
目的探讨重组幽门螺杆菌过氧化氢酶(rhpcat)对大鼠结肠粘膜上皮细胞氧化应激的影响。方法在分离培养正常大鼠结肠粘膜上皮细胞的基础上,利用feso4+h2o2反应产生羟自由基攻击制备结肠粘膜细胞氧化损伤模型,同时预先加入rhpcat并观察损伤减轻程度。实验设正常对照组、模型对照组、5-氨基水杨酸给药组(0.1mmol/l)、重组幽门螺杆菌过氧化氢酶给药组(1×105、1×106、1×107u/kg？b.w.)。培养一定时间后,取上清测乳酸脱氢酶(ldh)、丙二醛(mda)和髓过氧化物酶(mpo)的活性进行测定,并同时测定上述各组谷胱甘肽过氧化物酶(gsh-px)、过氧化氢酶(cat)和超氧化物歧化酶(sod)含量。结果模型组大鼠结肠粘膜上皮细胞mda、ldh和mpo水平增高,cat、gsh-px和sod含量减少。不同单位rhpcat呈剂量依赖性的减少ldh释放,抑制mda和mpo的形成,而使cat、gsh-px及sod恢复到正常水平。结论重组幽门螺杆菌过氧化氢酶对大鼠结肠粘膜氧化损伤具有保护作用。
| [1] | leroya,debruynegk,oomenlc,etal.alkylphospholipidsreversiblyopenepithelialtightjunctions[j].anticancerres,2003,23(1a):27-32.
|
| [2] | carrierj,aghdassie,platti,etal.effectoforalironsupplementationonoxidativestressandcolonicinflammationinratswithinducedcolitts[j].alimentpharmacolther,2001,15(12):1989-99.
|
| [3] | 余斌,覃承诃,罗吉伟,等.抗氧化维生素对大鼠运动能力的影响[j].第一军医大学学报,2003,23(9):892-4.yub,qinch,luojw,etal.effectofantioxidantvitaminsontheexerciseperformanceofrats[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2003,23(9):892-4.
|
| [4] | wangq,schererem,lemleyat.metribuzindegradationbymcmbraneanodicfentontreatmentanditsinteractionwithferricion[j].environscitechnol,2004,38(4):1221-7.
|
| [5] | 李国富,何一凡,黄木容,等.肺炎患者血清超氧化物歧化酶、谷胱甘肽过氧化物酶、总抗氧化能力、丙二醛的测定[j].第一军医大学学报,2003,23(9):961-2.ligf,heyf,huangmr,etal.determinationofserumsuperoxidedismutase,glutathioneperoxidase,totalantioxidativecapacity,malondialdehydeinpatientswithpneumonia[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2003,23(9):961-2.
|
| [6] | 徐强,徐如祥,张世忠,等.外源性超氧化物歧化酶保护6-羟基多巴所致大鼠中脑多巴胺能神经元凋亡的实验研究[j].第一军医大学学报,2003,23(11):1156-60.xuq,xurx,zhangsz,etal.protectiveeffectofexogenoussuperoxidedismutaseagainstratmidbraindopaminergicneuronapoptosisinducedby6-hydroxydopamine[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2003,23(11):1156-60.
|
| [7] | pencebc.dietaryseleniumandantioxidantstatus:toxiceffectsof1,2-dimethylhydrazineinrats[j].jnutr,1991,121(1):138-44.
|
| [8] | korkinal,suprunm,petrovaa,etal.theprotectiveandhealingeffectsofanaturalantioxidantformulationbasedonubiquinolandaloeveraagainstdextransulfate-inducedulcerativecolitisinrats[j].biofactors,2003,18(1-4):255-64.
|
| [9] | barbosads,cecchinir,el-kadrimz,etal.decreasedoxidativestressinpatientswithulcerativecolitissupplementedwithfishoilomega-3fattyacids[j].nutrition,2003,19(10):837-42.
|
| [10] | nieton,torresmi,fernandezmi,etal.experimentalulcerativecolitisimpairsantioxidantdefensesysteminratintestine[j].digdissci,2000,45(9):1820-27.
|
| [11] | 白杨,张亚历,王继德,等.幽门螺杆菌过氧化氢酶基因的克隆、高效表达及活性评价[j].中华消化杂志,2003,22(4):203-5.baiy,zhangyl,wangjd,etal.cloning,expressionandactivityofcatalasegeneofhelicobacterpylori[j].chinjdig,2003,22(4):203-5.
|
| [12] | 白杨,武金宝,王继德,等.幽门螺杆菌过氧化氢酶基因的克隆、序列测定及其生物信息学分析[j].微生物学免疫学进展,2003,31(3):1-4.baiy,wujb,wangjd,etal.cloning,sequencingandanalysisonthebiologicalinformationofcatalasegeneofhelicobacterpylori[j].progmicrobiolimmunol,2003,31(3):1-4.
|
| [13] | leschellex,robertv,delpals,etal.isolationofpigcoloniccryptsforcytotoxicassayofluminalcompounds:effectsofhydrogensulfide,ammonia,anddeoxycholicacid[j].cellbioltoxicol,2002,18(3):193-203.
|
| [14] | colletta,simse,walkerd,etal.comparisonofht29-18-c1andcaco-2celllinesasmodelsforstudyingintestinalparacellulardrugabsorption[j].pharmres,1996,13(2):216-21.
|
| [15] | venkatasubramanianj,sahij,raomc.iontransportduringgrowthanddifferentiation[j].annnyacadsci,2000,915(3):357-72.
|
| [16] | fontir,latellag,bisesg,etal.humancolonocytesinprimaryculture:amodeltostudyepithelialgrowth,metabolismanddifferentiation[j].ihtjcolorectaldis,1994,9(1):13-22.
|
| [17] | barena,sakamotok,shamsuddimam.long-termcultureofnormalhumancolonicepithelialcellsinvitro[j].fasebj,1992,9(6):2726-34.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
