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OALib Journal期刊
ISSN: 2333-9721
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巨噬细胞移动抑制因子对血管平滑肌细胞胶原ⅰ、ⅲmrna体外表达的影响
, PP. 684-686
Keywords: 巨噬细胞移动抑制因子,平滑肌细胞,胶原ⅰ,胶原ⅲ
Abstract:
目的探讨巨噬细胞移动抑制因子(mif)与血管平滑肌细胞(vsmc)合成胶原之间的可能联系。方法取大鼠主动脉中膜平滑肌进行原代细胞培养,用第4代传代细胞分为两组,用25、50、75、100ng/ml的mif刺激vsmc生长为实验组,未用刺激vsmc生长为实验组,采用rt-pcr,pcr方法测定vsmc胶原ⅰ、ⅲmrna含量。结果与正常对照组相比,除25ng/ml外,其他3种不同浓度的mif刺激smc,胶原ⅰmrna相对含量都显著增加(p<0.05)。100ng/ml的mif刺激smc,胶原ⅲmrna相对含量较对照组显著增加(p<0.05),而其余各浓度胶原ⅲmrna相对含量与对照组比较均无显著性差异。结论mif与vsmc合成胶原之间有关,mif刺激可以促进体外培养的vsmc胶原ⅰ、ⅲmrna表达。
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