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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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实时荧光聚合酶链反应检测hbv基因型b～d方法的建立
, PP. 630-632
Keywords: 肝炎病毒,乙型,基因型,实时荧光聚合酶链反应
Abstract:
目的建立实时荧光聚合酶链反应检测乙型肝炎病毒(hbv)基因型b~d方法并验证其准确性。方法比较genbank中已发表的明确分型的143株hbv全序列,设计特异的组合引物探针,应用实时荧光聚合酶链反应hbv基因分型法对兰州地区128例慢性乙型肝炎患者血清标本进行基因型b、c、d检测,并随机对检测的基因型各3株标本进行s基因测序验证。结果128例标本中b型检出率为20.3%(26/128),c型71.9%(92/128),d型7.8%(10/128);18株hbv克隆标本s基因测序结果与本分型法完全一致。结论实时荧光聚合酶链反应hbv基因分型法能够简便、灵敏、快速、准确地鉴定hbv基因型,适宜用于hbv基因型的大规模临床和流行病学调查。
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