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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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广州管圆线虫mr32000抗原的免疫诊断效果评价
, PP. 380-383
Keywords: 广州管圆线虫,抗原/纯化,免疫诊断
Abstract:
目的评价广州管圆线虫mr32000抗原(ac32)的诊断价值。方法利用切胶纯化法从广州管圆线虫成虫抗原中获取ac32,用westernblotting和elisa方法检测61例广州管圆线虫感染大鼠血清、5例正常鼠血清、1例广州管圆线虫病人血清、50例其他寄生虫体阳性患者血清和50例献血员血清。结果ac32和成虫粗抗原包被的elisa检测61份感染大鼠血清和1例临床诊断为广州管圆线虫病人血清均为阳性;ac32-elisa检测的50例献血员、20例急性血吸虫病人、11例弓形虫病人和所检测的其他寄生虫抗体阳性患者血清均为阴性。结论ac32在广州管圆线虫病的血清学诊断中具有较高的敏感性和特异性。
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