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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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重组丙型肝炎病毒在7721细胞中的复制
, PP. 387-390
Keywords: 丙型肝炎病毒,7721细胞系,逆转录-聚合酶链反应,共聚焦激光扫描显微镜
Abstract:
目的探讨构建的重组丙型肝炎病毒(hcv)能否在易感细胞系中复制和表达。方法用人工构建的hcv感染易感细胞系7721,培养72h后利用rt-pcr检测hcv的rna表达,并应用共聚焦激光扫描显微镜(clsm)观察免疫荧光标记的hcv核心抗原和胞膜抗原e1在细胞中的表达。结果人工构建的hcv感染7721细胞72h后,rt-pcr检测细胞内可检出hcv正链及负链rna,细胞培养上清中可检出hcv正链rna,clsm检测hcv核心抗原和e1抗原在细胞内的分布呈胞浆型。结论构建的hcv72h内可以在易感细胞系7721中复制和表达。
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