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抗hpv16e6核酶对宫颈癌caski细胞增殖和侵袭力的影响

, PP. 1367-1372

Keywords: 核酶,子宫颈癌,侵袭力,环氧合酶-2,血管内皮生长因子

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Abstract:

目的研究特异性抗hpv16e6核酶对宫颈癌caski细胞增殖和侵袭能力的影响。方法以脂质体法将抗hpv16e6核酶、空载体质粒分别导入caski细胞,分别为caski-r和caski-p。观察细胞的生长状态,测定caski,caski-r和caski-p3种细胞的生长曲线、软琼脂克隆形成率、细胞侵袭力实验、裸鼠体内致瘤性,以及rt-pcr检测细胞中cox-2、血管内皮生长因子(vegf)的表达,同时,免疫组化检测3种细胞cox-2、vegf抗原表达,分析特异性抗hpv16e6核酶对宫颈癌caski细胞增殖和侵袭力的影响。结果caski、caski-p细胞生长速率、软琼脂克隆形成率、致瘤性和侵袭力相近,而caski-r细胞的细胞生长速率、软琼脂克隆形成率、致瘤性和侵袭力与以上两种细胞相比却明显降低。rt-pcr检测caski-r细胞的cox-2、vegf表达水平也低于caski、caski-p细胞的表达。细胞免疫组化测定caski、caski-p和caski-r细胞都有cox-2、vegf抗原表达,而caski-r细胞的抗原染色程度较弱。结论特异性抗hpv16e6核酶的导入降低了宫颈癌caski细胞的增殖和侵袭表型,可能与宫颈癌caski细胞内cox-2、vegf的表达水平降低有关。

References

[1]  zhengyf,zhangjr,qulh,etal.effectivenessofaribozymeforcleavageofanrnatranscriptfromhumanpapillomavirustype16[j].intjmoderncancerther,1998,1(1):134-8.
[2]  郑燕芳,饶智国,张积仁.抗hpv16e6核酶对宫颈癌细胞恶性表型的影响[j].中国肿瘤生物治疗杂志,2002,9(3):183-5.zhengyf,raozg,zhangjr.theeffectofanti-hpv16e6-ribozymeonmalignantphenotypesofcervicalcarcinomacellline[j].chinjcancerbiother,2002,9(3):183-5.
[3]  tokumok,kodamaj,sekin,etal.differentangiogenicpathwaysinhumancervicalcancers[j].gynecoloncol,1998,68(1):38-44.
[4]  santinad,hermonatpl,ravaggia,etal.secretionofvascularendothelialgrowthfactorinadenocarcinomaandsquamouscellcarcinomaoftheuterinecervix[j].obstetgynecol,1999,94(1):78-82.
[5]  uedam,teraiy,yamashitay,etal.correlationbetweenvascularendothelialgrowthfactor-cexpressionandinvasionphenotypeincervicalcarcinomas[j].intjcancer,2002,98(3):335-43.
[6]  tsujiim,duboisrn.alterationsincellularadhesionandapoptosisinepithelialcellsoverexpressingprostaglandinendoperoxidesynthase2[j].cell,1995,83(3):493-501.
[7]  jaimelm,kathleenml,manetk,etal.antiangiogenticandantitumorofcyclooxygenas-2inhhibitors[j].cancerres,2000,60:1306-11.
[8]  饶智国,张积仁,郑燕芳,等.抗hpv16e6核酶对宫颈癌caski细胞化疗的影响[j].第一军医大学学报,2002,22(4):316-9raozg,zhangjr,zhengyf,etal.effectofanti-hpv16e6-ribozymeonthesensitivityofcervicalcarcinomacelllinetochemotherapy[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2002,22(4):316-9.
[9]  alanirm,mungerk.humanpapillomavirusandassociatedmalignancies[j].jclinoncol,1998,16(1):330-7.
[10]  郑燕芳,张积仁,屈良鹄,等.两种抗hpv核酶的设计、表达与活性鉴定[j].中华实验与临床病毒学杂志,1999,13(2):238,242.zhengyf,zhangjr,qulh,etal.thedesign,expressionandactivityevaluationoftwokindsofanti-hpvribozyme[j].chinjexpclinvirol,13(2):238,242.
[11]  郑燕芳,饶智国,张积仁.特异性核酶对宫颈癌细胞系caski增殖与凋亡的影响[j].第一军医大学学报,2002,22(6):496-502.zhengyf,raozg,zhangjr.effectsofanti-hpv16e6-ribozymeontheproliferationandapoptosisofhumancervicalcancercelllinecaski[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2002,22(6):496-502.
[12]  albinia,iwamotoy,kleinmanhk,etal.arapidinvitroassayforquantitatingtheinvasivepotentialoftumorcells[j].cancerres,1987,47(12):3239-45.
[13]  chenz,kamathp,zhangs,etal.effectsontumorcellsofribozymesthatcleavethernatranscriptsofhumanpapillomaviustype18[j].cancergenetherapy,1996,3(1):18-23.

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