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OALib Journal期刊
ISSN: 2333-9721
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七种常见mbl基因单倍型标准质粒的构建
, PP. 1379-1383
Keywords: 甘露聚糖结合凝集素,单核苷酸多态性,单倍型,标准质粒
Abstract:
目的构建7种常见的mbl基因单倍型标准质粒。方法以已确认mbl基因单倍型及基因型的dna样本为模板,利用ssp-pcr技术扩增出包含mbl基因启动子区及第一外显子的单倍型片段并将其克隆入t载体;用定点突变技术分别将其第一外显子52和57位密码相应的碱基突变;采用pcr和测序进行鉴定。结果以单倍型及基因型已确认的dna样本为模板,采用ssp-pcr和分子克隆技术获得单倍型hypa、lxpa、lyqa、lypa和lypb重组质粒;以单倍型hypa及lyqa重组质粒为模板,采用定点突变技术获得单倍型hypd及lyqc标准质粒。结论构建成功常见的7种mbl基因单倍型标准质粒,为采用ssp-pcr、real-timepcr等技术分析mbl基因相应的snp及其单倍型与基因型提供了标准对照。
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