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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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血清蛋白质组学研究中白蛋白和igg去除方法的建立与评价
, PP. 1233-1235
Keywords: 血清蛋白质组学,双向凝胶电泳,白蛋白,igg
Abstract:
目的建立和评价血清蛋白质组学研究中白蛋白和igg的去除方法。方法应用microbio-spincolumn过滤纯化血清蛋白,去除白蛋白和igg,利用双向凝胶电泳(2-de)分析评价血清蛋白过滤效果。结果建立了血清白蛋白和igg去除方法;过滤后大部分血清白蛋白和igg能够去除,2-de图谱中低丰度蛋白质分辨率增加。结论本研究建立的血清蛋白质纯化技术,可有效应用于疾病的血清蛋白质组学研究。
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