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OALib Journal期刊
ISSN: 2333-9721
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环孢菌素a对体外nit-1胰岛β细胞增殖及其增殖相关基因表达的影响
, PP. 1278-1280
Keywords: 环孢菌素a,细胞增殖/药物作用,rt-pcr,nit-1细胞
Abstract:
目的观察环孢霉素a(csa)对体外培养的nit-1胰岛β细胞增殖和polα1mrna表达的影响。方法用mtt法检测不同浓度的csa(0.05~10μmol/l)作用48和72h后nit-1胰岛β细胞增殖的情况。利用半定量rt-pcr法检测10μmol/lcsa处理nit-1胰岛β细胞48h后polα1在mrna水平上的表达。结果csa可抑制nit-1胰岛β细胞的增殖,且与时间、剂量正相关。另外,csa还下调了polα1mrna的表达。结论csa可抑制nit-1胰岛β细胞增殖,其机制可能与下调polα1mrna表达有关。
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