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OALib Journal期刊
ISSN: 2333-9721
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分子信标探针技术检测沙门菌inva基因
, PP. 1257-1259
Keywords: 沙门菌,分子信标,inva基因
Abstract:
目的研究沙门菌的分子信标基因检测方法。方法在pcr反应体系中加入分子信标探针,探针的5’端标记6-fluorescine(6-fam),3’端标记4-(dimethylaminophenylazo)benzoicacid(dabcyl),对沙门菌inva基因pcr产物进行荧光检测。结果肠炎沙门菌、甲型副伤寒沙门菌、乙型副伤寒沙门菌、伤寒沙门菌“h”、伤寒沙门菌和肠侵袭型大肠杆菌的荧光值分别为161.6、104.5、85.9、83.1、94.8、46.1,ax值(样本荧光值-空白对照荧光值)分别为121.3、64.2、45.6、42.8、54.5、5.8,沙门菌的ax值均大于21,结果阳性,与琼脂糖电泳分析结果一致。结论分子信标探针技术可以准确、快速、简便进行沙门菌inva基因检测。
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