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OALib Journal期刊
ISSN: 2333-9721
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应用rna干扰抑制eb病毒潜伏膜蛋白-1表达对鼻咽癌细胞生长的影响
, PP. 241-246
Keywords: 鼻咽癌,eb病毒,潜伏膜蛋白-1,rna干扰
Abstract:
目的探讨能否通过sirna人工诱导rna干扰,在eb病毒阳性的鼻咽癌细胞株c611中,选择性抑制潜伏膜蛋白1(lmp1)的表达,并了解lmp1抑制对鼻咽癌细胞生长的影响。方法利用脂质体转染的方法,将人工合成的双链sirna导入鼻咽癌细胞,通过半定量rt-pcr检测lmp1mrna水平的变化。并采用mtt法和流式细胞仪检测lmp1表达抑制后,鼻咽癌细胞增殖和细胞周期的变化。结果c611细胞转染sirna可使lmp1mrna水平下降90%以上。lmp1基因抑制后,c611细胞增殖速度下降33%;流式细胞检测显示,c611细胞周期在g0-g1期受阻。结论本研究证明,全新的人工诱导rna干扰技术,能够有效抑制lmp1基因的表达,并降低鼻咽癌细胞的增殖能力,为鼻咽癌研究和抗肿瘤基因治疗提供了新的思路。
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