|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
sars冠状病毒n基因的扩增与克隆
, PP. 39-41
Keywords: 严重急性呼吸综合征,n基因,冠状病毒,逆转录-聚合酶链反应
Abstract:
目的rt-pcr扩增、克隆sars冠状病毒n蛋白基因。方法根据genbank数据库中tor2株的全基因组序列,利用primerpremier5.0软件设计引物rt-pcr巢式扩增sars冠状病毒的n基因,pcr产物克隆后进行测序鉴定。结果序列分析表明,pmd18-t载体中已成功重组了n基因。结论n蛋白基因的扩增、克隆成功,为n蛋白的表达、n蛋白结构与功能的研究奠定了基础。
| [1] | peirisj,lais,poonl,etal.coronavirusasapossiblecauseofsevereacuterespiratorysyndrome[j].lancet,2003,361(9366):1319-25.
|
| [2] | ksiazektg,erdmand,goldsmithcs,etal.anovelcoronavirusassociatedwithsevereacuterespiratorysyndrome[j].nengljmed,2003,348(20):1953-66.
|
| [3] | rotapa,oberstems,monroess,etal.characterizationofanovelcoronavirusassociatedwithsevereacuterespiratorysyndrome[j].science,2003,300(5624):1394-9.
|
| [4] | nelsongw,stohlmansa,taharasm.highaffinityinteractionbetweennucleocapsidproteinandleader/intergenicsequenceofmousehepatitisvirusrna[j].jgenvirol,2000,81(pt1):181-8.
|
| [5] | chenh,wurmt,brittonp,etal.interactionofthecoronavirusnucleoproteinwithnucleolarantigensandthehostcell[j].jvirol,2002,76(10):5233-50.
|
| [6] | 王艳,马文丽,宋艳彬,等.反转录巢式pcr方法检测sars冠状病毒[j].第一军医大学学报,2003,23(5):421-3.wangy,mawl,songyb,etal.genesequenceanalysisofsars-associatedcoronavirusbynestedrt-pcr[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2003,23(5):421-3.
|
| [7] | 车小燕,丘立文,潘玉先,等.sars冠状病毒n蛋白单克隆抗体的快速、高效制备的方法研究[j].第一军医大学学报,2003,23(7):640-2.cbexy,qiulw,panyx,etal.rapidandefficientpreparationofmonoclonalantibodiesagainstsars-associatedcoronavirusnucleocapsidproteinbyimmunizingmice[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2003,23(7):640-2.
|
| [8] | 车小燕,郝卫,丘立文,等.sars病人sars冠状病毒核壳抗原抗体的变化规律[j].第一军医大学学报,2003,23(7):637-9.chexy,haow,qiulw,etal.antibodyresponseofpatientswithsevereacuterespiratorysyndrome(sars)tonucleocapsidantigenofsars-associatedcoronavirus[j].jfirstmilmeduniv/diyijunyidaxuexuebao,2003,23(7):637-9.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
