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OALib Journal期刊
ISSN: 2333-9721
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抗sars冠状病毒s1蛋白n端249至667的单克隆抗体的制备与鉴定
, PP. 1-6
Keywords: sars冠状病毒,单克隆抗体,刺突蛋白,受体结合区
Abstract:
目的在获得了具有免疫原性的sars冠状病毒s1蛋白片段的基础上,制备和鉴定特异性抗该段s1蛋白单克隆抗体(mab)。方法原核表达含s蛋白受体结合区的sars冠状病毒s1蛋白片段s1c(n端249-667氨基酸残基),其免疫原性经sars病人恢复期血清鉴定后免疫balb/c小鼠,按常规方法制备单克隆抗体,并采用elisa间接法、免疫荧光和免疫印迹进行筛选和鉴定。结果筛选出3株特异性针对sars冠状病毒s1蛋白n端249-667的mab杂交瘤细胞株,igg亚类鉴定1株为igg1,2株为igg2a,经免疫荧光鉴定与人冠状病毒株229e和oc43无交叉反应。结论获得3株抗sars冠状病毒s蛋白受体结合区特异性单克隆抗体,为建立新的sars冠状病毒检测方法的和进一步研究s蛋白的功能奠定了基础。
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