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3号染色体短臂21-22区域63个新基因在鼻咽癌组织中的差异表达

Keywords: 鼻咽癌,3p21-22,基因表达,染色体

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Abstract:

目的寻找与鼻咽癌发病密切相关的未知基因。方法对63个定位于3号染色体短臂21-22d3s1609-d3s1295区域的单拷贝并代表未知基因的est簇进行网上克隆,在获取基因大片段或全长cdna序列的基础上,应用半定量逆转录-聚合酶链反应检测63个未知基因在4种鼻咽癌细胞株、32例鼻咽部低分化鳞状细胞癌组织和16例鼻咽部慢性炎症组织中的表达状况。结果49个未知基因在鼻咽癌组织和鼻咽部慢性炎症组织间的表达水平无显著性差异;8个未知基因在鼻咽癌和鼻咽部慢性炎症组织中均不表达;2个未知基因在鼻咽癌组织中表达明显增强;4个未知基因在鼻咽癌组织中表达明显减弱,其中hs.27566基因在鼻咽癌细胞和组织中均显著低表达或不表达。结论在染色体3p21-22d3s1609-d3s1295区域构建了63个未知基因在鼻咽癌的表达差异谱;筛选出6个在鼻咽癌组织中差异表达的未知基因。

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