|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
人大肠癌线粒体dna重组质粒的构建
, PP. 1016-1019
Keywords: 大肠癌,线粒体,dna,d-环区,突变,质粒
Abstract:
目的了解大肠癌细胞株(sw480,lovo,ht29)线粒体dna的突变,克隆突变的大肠癌线粒体dna(mtdna)基因,构建pcdna3.i(+)-mtdna真核表达重组体,并导入nih3t3细胞,以探讨线粒体基因突变与肿瘤发生的关系。方法提取大肠癌细胞株(sw480,lovo,ht29)mtdna,扩增d-loop区,产物用dna自动测序法进行序列分析。利用dna重组技术将其定向插人真核表达质粒pcdna3.1(+),并用脂质体法导人nih3t3细胞。结果检测出大肠癌细胞株sw480、lovo、ht29细胞mtdnad-loop分别有10、9、8个突变位点。成功克隆1119bp的mtdnad-loop区至表达质粒pcdna3.1(+),并导入nih3t3细胞中。结论线粒体dnad-loop区是一个具有高度多态性和突变性的区域,在大肠癌细胞株中突变率较高。
| [1] | jerryws,haroldw.aremitochondrialdnamutationsinvolvedinthecarcinogenicprocess[j]?mutatres,1987,186(10):149-60.
|
| [2] | lucianerc,bertrandcl.mutagenesis,tumorigenicityandapoptosis:arethemitochondriainvolved[j]?mutatres,1998,398:19-26.
|
| [3] | millarcb,guyj,sansomoj,etal.enhancedcpgmutabilityandtumorigenesisinmbd4-deficientmice[j].science,2002,297(5):403-5.
|
| [4] | matsudat,vandebergbj,bebenekk,etal.thebasesubstitutionfidelityofdnapolymerasebeta-dependentsinglenucleotidebaseexcisionrepair[j].jbiolchem,2003,278(5):25947-51.
|
| [5] | servantl,bietha,hayakawah,etal.involvementofdnapolymerasebetaindnareplicationandmutagenicconsequences[j].jmolbiol,2002,315(10):1039-47.
|
| [6] | takeuchih,fujimotoa,hoonds.detectionofmitochondrialdnaalterationsinplasmaofmalignantmelanomapatients[j].annnyacadsci,2004,1022(8):50-4.
|
| [7] | solanoa,roigm,vives-bauzac,etal.bilateralstriatalnecrosisassociatedwithanovelmutationinthemitochondrialnd6gene[j].annneurol,2003,54(1):527-30.
|
| [8] | liucy,leecf,hongch,etal.mitochondrialdnamutationanddepletionincreasethesusceptibilityofhumancellstoapoptosis[j].annnyacadsci,2004,101(6):133-45.
|
| [9] | abnetcc,huppik,carreraa,etal.controlregionmutationsandthe’commondeletion’arefrequentinthemitochondrialdnaofpatientswithesophagealsquamouscellcarcinoma[j].bmccancer,2004,4(2):27-30.
|
| [10] | berettas,mattavellil,salag,etal.leberhereditaryopticneuropathymtdnamutationsdisruptglutamatetransportincybridcelllines[j].brain,2004,127(10):2183-92.
|
| [11] | makikosf,henningu,otavialc,etal.faciledetectionofmitochondrialdnamutationsintumorandbodilyfluids[j].science,2000,287(6):2017-9.
|
| [12] | habanow,sugait,nakamrasi,etal.microsatelliteinstabilityandmutationofmitochondrialandnucleardnaingastriccarcinoma[j].gastroenterology,2000,118(11):835-41.
|
| [13] | 王平忠,蒙世杰,刘佩,等.大鼠乳腺肿瘤线粒体基因突变研究[j].中国病理生理杂志,2001,17(1):297-301.
|
| [14] | habanow,sugait,nakamurasi,etal.microsatelliteinstabilityandmutationofmitochondrialandnucleardnaingastriccarcinoma[j].gastroenterology,2000,118(5):835-41.
|
| [15] | tongbc,hapk,dhirk,etal.mitochondrialdnaalterationsinthyroidcancer[j].jsurgoncol,2003,82(1):170-3.
|
| [16] | zhous,kachhaps,singhkk.mitochondrialimpairmentinp53-deficienthumancancercells[j].mutagenesis,2003,18(10):287-92.
|
| [17] | hanselde,kernse,hrubanrh.molecularpathogenesisofpancreaticcancer[j].annurevgenomicshumgenet,2003,4(10):237-56.
|
| [18] | wonglj,tandj,bairk,etal.molecularalterationsinmitochondrialdnaofhepatocellularcarcinomas:isthereacorrelationwithclinicopathologicalprofile[j]?jmedgenet,2004,41(12):59-65.
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
