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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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斑蝥素对g1/s期肾小管上皮细胞atp的调节
Keywords: 肾小管上皮细胞,斑蝥素,三磷酸腺苷,肌动蛋白类,低氧
Abstract:
目的通过斑蝥素对g1/s期肾小管上皮细胞(rtecs)低氧损伤后细胞atp代谢影响的研究,探讨斑蝥素稳定肌动蛋白微丝骨架系统的机制。方法将新生猪g1/s界面rtecs分为3组:实验组(氰化钠+斑蝥素)、缺氧组(氰化钠)和空白对照组,应用反相高效液相比较缺氧后各组细胞内atp含量的变化。结果实验组细胞内atp含量[(14.50±0.26)mmol/g蛋白]明显高于缺氧组[(4.25±0.11)mmol/g蛋白]和空白对照组[(8.58±0.13)mmol/g蛋白](p<0.01)。结论斑蝥素稳定肌动蛋白微丝骨架系统可能与抗rtecs低氧损伤后细胞能量代谢紊乱相关。
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