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OALib Journal期刊
ISSN: 2333-9721
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副溶血弧菌不耐热溶血毒素tlh基因的序列分析
, PP. 1153-1155
Keywords: 弧菌,副溶血性,碱基序列,聚合酶链反应,溶血素类
Abstract:
目的利用pcr技术扩增副溶血弧菌不耐热溶血毒素tlh基因,对tlh基因进行生物信息学分析。方法利用pcr技术扩增tlh基因,克隆至载体pet32a+并测序。将测序结果提交国际互联网上有关生物信息网站进行生物信息学分析。结果和结论测序结果tlh14-90被genbank收录,登录号为ay289609。tlh14-90全长1257bp,与国际标准株wp1的同源性为99%,含有启动子和密码子,预测其编码一个含418个氨基酸的蛋白质(tlh14-90),分子式为c2131h3184n548o649s16,相对分子质量47392.9,理论等电点pi为4.92,丙氨酸、亮氨酸和天冬酰氨的含量分别为11.0%、7.4%和7.2%,含有4个半胱氨酸(cys),κ-d法推算的疏水性参数为-34。预测其二级折叠结构为α/□蛋白,三级结构未给出。
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