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OALib Journal期刊
ISSN: 2333-9721
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利用绿色荧光蛋白筛选酵母突变株的新方法
, PP. 1150-1152
Keywords: 同源重组,转座子,绿色荧光蛋白,酵母,突变株
Abstract:
目的建立快速简便筛选酵母突变株的方法。方法利用同源重组技术,通过转座子上的gfp基因,从酵母基因组文库中,检测发生突变的菌株,从而筛选出酵母突变株。结果应用荧光显微镜观察sc-ura选择平板筛选出的酵母,有绿色荧光发出的即为阳性菌落。结论利用同源重组技术,初步筛选出了所需的酵母突变株。
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